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Series GSE23534 Query DataSets for GSE23534
Status Public on Aug 11, 2010
Title Effect of hIAPP aggregation on gene expression by mouse bone marrow-derived macrophages after 12 hours
Organism Mus musculus
Experiment type Expression profiling by array
Summary The purpose of this experiment was to determine changes in gene expression by bone marrow-derived macrophages (BMDMs) treated with synthetic human vs. rodent islet amyloid polypeptide (IAPP). Synthetic human IAPP at 15 uM aggregates to form fibrils in vitro, whereas rodent IAPP is non-amyloidogenic. We hypothesized that interaction of macrophages with human IAPP aggregates can activate pro-inflammatory signalling pathways in macrophages, as described for other amyloidogenic peptides.
Overall design This array includes eight samples from one experiment, with two groups of four replicates each. Each replicate represents BMDMs from one well of a 24-well plate. The control group was treated with 15 uM rat IAPP for 12 hours prior to total RNA extraction; the experimental group was treated with 15 uM human IAPP.
Contributor(s) Westwell-Roper C, Verchere CB
Citation(s) 21813778
Submission date Aug 10, 2010
Last update date Jun 14, 2018
Contact name Clara Westwell-Roper
Phone 604-875-3792
Organization name Child and Family Research Institute, University of British Columbia
Department Pathology and Laboratory Medicine
Lab Dr. C. Bruce Verchere
Street address 950 West 28th Ave
City Vancouver
State/province BC
ZIP/Postal code V5Z 4H4
Country Canada
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM577091 macrophage_rIAPP15uM_12h_rep1
GSM577092 macrophage_rIAPP15uM_12h_rep2
GSM577093 macrophage_rIAPP15uM_12h_rep3
BioProject PRJNA131753

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23534_RAW.tar 3.1 Mb (http)(custom) TAR
GSE23534_non-normalized.txt.gz 937.8 Kb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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