|
| Status |
Public on Sep 21, 2010 |
| Title |
Identification of immediate early transcriptional targets of ephrin-B1 forward signaling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The goal of this study was to identify immediate early transcriptional targets of ephrin-B1 forward signaling that are relevant to palatogenesis.
The Ephs compose a family of receptor tyrosine kinase signaling molecules that can be activated by their cognate ligands, the ephrins. Despite the importance of Eph/ephrin signaling in a wide variety of developmental and cell biological processes, a potential downstream transcriptional response has not been explored. To understand the role of ephrin-B1 signaling in palatogenesis, we examined transcriptional response to ephrin-B1 in a primary mouse embryonic palatal shelf cell culture system. We find an immediate early signature of gene expression that reflects the activation of Erk/MAPK signaling by ephrin-B1 signaling in the palatal shelves.
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| |
|
| Overall design |
Induction of primary palate cells with 2 ug/ml of pre-clustered ephrin-B1 for one hour. Uninduced primary palate cells are the control. Four replicates each.
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| |
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| Contributor(s) |
Bush JO, Soriano P |
| Citation(s) |
20844017 |
| Submission date |
Aug 10, 2010 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Jeffrey Ohmann Bush |
| E-mail(s) |
jeffrey.bush@mssm.edu, johmannbush@gmail.com
|
| Organization name |
Mount Sinai School of Medicine
|
| Department |
Developmental and Regenerative Biology
|
| Lab |
Soriano
|
| Street address |
One Gustave Levy Place, Box 1020
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6105 |
Illumina mouse-6 v1.1 expression beadchip |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA131747 |
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