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| Status |
Public on May 11, 2011 |
| Title |
Conversion of mouse fibroblast to hepatocyte-like cells by defined factors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Plasticity of differentiated cells has been proved by nuclear transfer, induced pluripotent cells and transdifferentiation. Here we show that by transduction of 3 factors (Hnf1alpha, Gata4, and Foxa3) and p19Arf inactivation, tail-tip fibroblasts can be converted to hepatocyte-like (iHep) cells, expressing hepatocyte marker genes, and acquiring many mature hepatocyte functions in vitro and in vivo.
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| Overall design |
p19Arf-null TTFs were tranfected with 3 liver enriched transcription factors, then changed to modified Block's medium. To enrich iHep cells, epithelial cells were enriched by partial trypsin digestion.
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| Contributor(s) |
Huang P, Hui L |
| Citation(s) |
21562492 |
| Submission date |
Aug 16, 2010 |
| Last update date |
May 10, 2018 |
| Contact name |
Lijian Hui |
| E-mail(s) |
lidan@sibcb.ac.cn
|
| Phone |
+86-21-54921328
|
| Organization name |
Shanghai Institutes for Biological Sciences, Chinese Academy for Sciences
|
| Department |
Institute of Biochemistry and Cell Biology
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| Lab |
Laboratory of Molecular Cell Biology
|
| Street address |
Yueyang Road 320
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA130939 |
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