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Status |
Public on Aug 13, 2012 |
Title |
COMPARATIVE TRANSCRIPTOMIC AND PROTEOMIC ANALYSIS OF LGR5+ve STEM CELLS AND THEIR DAUGHTERS (AGILENT ARRAYS) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
The identification of Lgr5 as an intestinal stem cell marker has made it possible to isolate and study primary intestinal stem cells. Applying quantitative mass spectrometry as well as transcriptomic analysis, we profiled the protein and gene changes between FACS-sorted Lgr5+ve stem cells and their immediate undifferentiated daughter cells. The overall comparison of mRNA and protein levels revealed a high level of correlation, implying that the initial control of intestinal stem cell biology occurs largely at the mRNA level. Taken together, our study presents a valuable resource for the study of intestinal stem cell biology.
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Overall design |
We used cell fractions of intestines from Lgr5-EGFP-ires-CreERT2 mice, expressing GFP under the control of the Lgr5 promoter. RNA was isolated from two FACS sorted cell populations, one expressing GFP at high levels (GFPhi) and the other expressing GFP at low levels (GFPlow). The gates set to sort cells for the expression profiling were the same as for the cells used for the mass spectrometry analysis. Differentially labelled cRNA from GFPhi and GFPlow cells from two different sorts (each combining three different mice) were hybridized on 4X44K Agilent Whole Mouse Genome dual colour Microarrays (G4122F) in two dye swap experiments, resulting in four individual arrays.
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Contributor(s) |
Stange DE, van de Wetering M, Clevers H |
Citation(s) |
22855427, 22692129 |
Submission date |
Aug 17, 2010 |
Last update date |
May 10, 2018 |
Contact name |
Daniel E. Stange |
E-mail(s) |
daniel.stange@uniklinikum-dresden.de
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Organization name |
University Clinic Dresden
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Street address |
Fetscherstr. 74
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City |
Dresden |
ZIP/Postal code |
01307 |
Country |
Germany |
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Platforms (1) |
GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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Samples (4)
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This SubSeries is part of SuperSeries: |
GSE33949 |
COMPARATIVE TRANSCRIPTOMIC AND PROTEOMIC ANALYSIS OF LGR5+ve STEM CELLS AND THEIR DAUGHTERS |
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Relations |
BioProject |
PRJNA155475 |