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| Status |
Public on Aug 31, 2010 |
| Title |
Progressive lengthening of 3' untranslated regions of mRNAs by alternative polyadenylation during C2C12 differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The 3' untranslated regions (3' UTRs) of mRNAs contain cis-acting elements for posttranscriptional regulation of gene expression. Here, we report that mouse genes tend to express mRNAs with longer 3' UTRs as embryonic development progresses. This global regulation is controlled by alternative polyadenylation and coordinates with initiation of organogenesis and aspects of embryonic development, including morphogenesis, differentiation, and proliferation. Using myogenesis of C2C12 myoblast cells as a model, we recapitulated this process in vitro and found that 3' UTR lengthening is likely caused by weakening of mRNA polyadenylation activity. Because alternative 3' UTR sequences are typically longer and have higher AU content than constitutive ones, our results suggest that lengthening of 3' UTR can significantly augment posttranscriptional control of gene expression during embryonic development, such as microRNA-mediated regulation.
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| Overall design |
Two biological replicates of C2C12 growth and differentiation conditions, repesctively
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| Contributor(s) |
Ji Z, Tian B |
| Citation(s) |
19372383 |
| Submission date |
Aug 30, 2010 |
| Last update date |
Mar 06, 2018 |
| Contact name |
Zhe Ji |
| E-mail(s) |
zhe.ji@northwestern.edu
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| Organization name |
Northwestern University
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| Street address |
303 E Superior St, Lurie 6-107
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| City |
Chicago |
| State/province |
ILLINOIS |
| ZIP/Postal code |
60611 |
| Country |
USA |
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| Platforms (1) |
| GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| GSM588561 |
C2C12 differentiation condition, rep2 |
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| Relations |
| BioProject |
PRJNA130597 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23871_RAW.tar |
87.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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