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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 20, 2011 |
| Title |
Genome-wide analysis of gene expression regulated by nucleolin in mouse embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To further explore the function and underlying mechanism of nucleolin in embryonic stem cells (ESCs), we compared the transcription profile of tetracycline (Tc)-inducible EGFP (as a control) or nucleolin siRNA expression-stable ESCs cultured with or without Tc for 3 days, and identified distinct classes of up- and down-regulated genes by nucleolin in mouse ESCs.
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| Overall design |
Total RNA obtained from CGR8 ES cells stably integrated with Tc-inducible siRNA EGFP, nucleoin-1 or nucleolin-2 cultured with or without Tc for 3 days, and then hybridized to Illumina Sentrix Mouse-6 v2.0 BeadChips. Three replicates each condition.
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| Contributor(s) |
Yang A, Jin Y |
| Citation(s) |
22013067 |
| Submission date |
Sep 07, 2010 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Ying Jin |
| E-mail(s) |
yjin@sibs.ac.cn
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| Phone |
86-21-63852591
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| Fax |
86-21-63852591
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| Organization name |
Shanghai Jiao Tong University School of Medicine
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| Department |
Shanghai Stem Cell Institute
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| Street address |
225 South Chongqing Road
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| City |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (18)
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| GSM591039 |
siRNA EGFP +Tc, rep1 |
| GSM591040 |
siRNA EGFP +Tc, rep2 |
| GSM591041 |
siRNA EGFP +Tc, rep3 |
| GSM591042 |
siRNA Ncl-1 -Tc, rep1 |
| GSM591043 |
siRNA Ncl-1 -Tc, rep2 |
| GSM591044 |
siRNA Ncl-1 -Tc, rep3 |
| GSM591045 |
siRNA Ncl-1 +Tc, rep1 |
| GSM591046 |
siRNA Ncl-1 +Tc, rep2 |
| GSM591047 |
siRNA Ncl-1 +Tc, rep3 |
| GSM591048 |
siRNA Ncl-2 -Tc, rep1 |
| GSM591049 |
siRNA Ncl-2 -Tc, rep2 |
| GSM591050 |
siRNA Ncl-2 -Tc, rep3 |
| GSM591051 |
siRNA Ncl-2 +Tc, rep1 |
| GSM591052 |
siRNA Ncl-2 +Tc, rep2 |
| GSM591053 |
siRNA Ncl-2 +Tc, rep3 |
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| Relations |
| BioProject |
PRJNA130283 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23993_RAW.tar |
15.8 Mb |
(http)(custom) |
TAR |
| GSE23993_non-normalized.txt.gz |
5.4 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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