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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 31, 2010 |
| Title |
Investigation of extra-telomeric effects of hTERT in neoplastic transformation of IMR90 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The complexity and heterogeneity of cancer cells are under-represented by the current experimental models which revolve around primary tumor cells, cancer cell lines, and rodent models. We used the transformed cell model in our studies. IMR90 and BJ fibroblast cells were transformed by three oncogenic genetic factors, SV40 Large-T antigen, H-Ras and human telomerase (hTERT). These transformed cells can grow under anchorage independent conditions, are self-sufficient in growth signals, have decreased sensitivity to apoptosis, and showed recurrent chromosomal abnormalities. Further characterization of transformed cells through cell cycle profiling and mass spectrometry analysis also revealed an increased fraction in the G2/M phase, and an up-regulation of Ku70. Furthermore, transformed cells exhibited increased telomerase activity that was not accountable by hTERT overexpression alone. Microarray results revealed that hTERT overexpression promoted cell migration and the initiation of DNA damage responses; two cellular processes that are important in cancer progression. Collectively, these data imply that IMR90 transformed cell model is an invaluable tool for cancer studies. Our results in the systematic study of this model helped us identify possible early events in cancer transformation and reveal the extra-telomeric effects of telomerase in cell migration and DNA damage initiation.
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| Overall design |
IMR90 and BJ fibroblast cells were transformed by three oncogenic genetic factors, SV40 Large-T antigen, H-Ras and human telomerase (hTERT). Through microarray analysis, gene expression of transformed cells were compared against control cells.
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| Contributor(s) |
Mahalingam D, Luo Z, Tan WH, Huang X, Lim YP, Deng L, Cacheux-Rataboul V, Liu ET, Blackburn E, Wang X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 13, 2010 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Tan Wei Han |
| Organization name |
National University of Singapore
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| Street address |
21 Lower Kent Ridge Road
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| City |
Singapore |
| ZIP/Postal code |
119077 |
| Country |
Singapore |
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| Platforms (1) |
| GPL6104 |
Illumina humanRef-8 v2.0 expression beadchip |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA130297 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24097_RAW.tar |
3.4 Mb |
(http)(custom) |
TAR |
| GSE24097_additional_info.txt.gz |
786 b |
(ftp)(http) |
TXT |
| GSE24097_non-normalized.txt.gz |
2.1 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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