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Series GSE24213 Query DataSets for GSE24213
Status Public on Mar 23, 2015
Title Expression profiling of rat heart and blood after amiodarone treatment
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary In the present study, an oligonucleotide microarray platform was used to compare expression profiles of heart and blood in rats treated with amiodarone against respective controls. Male Sprague-Dawley (CD) rats, which were 10 weeks old and weighed 290-320 grams at the beginning of the study, have been treated for ten days with amiodarone by oral route at a dose of 300mg/Kg/die. The study has been constituted by a control group, which received the vehicle (methylcellulose 1%, w/v), and by a treatment group (10 animals for both groups). For each tissue sample, total RNA has been individually extracted and evaluated. For each tissue, three pools/group (2 pools composed of 3 animals and the last one by 4) have been used for microarray analysis. A modulation of 600 genes in heart and 8300 genes in blood has been obtained.
 
Overall design In this study, we analyzed the gene expression profiles of 6 pools (3 composed of control rats and 3 composed of rats treated with amiodarone) for each tissue (blood and heart) using the Agilent-014879 Rat oligo microarray platform (12 arrays, no replicates) based on single-colour detection (Cyanine-3 only). Microarrays were scanned with Agilent scanner G2565BA (barcode on the left, DNA on the back surface, scanned through the glass) at a resolution of 5 microns; all slides were scanned twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%); the scanner software creates a unique ID for each pair of XDR scans and saves it to both scan image files. Feature Extraction 9.5 uses XDR ID to link the pairs of scans together automatically when extracting data. The signal remaining, after all the FE processing steps have been completed, is ProcessedSignal that contains the Multiplicatively Detrended, Background-Subtracted Signal. The heart and blood samples were normalized separately.
 
Contributor(s) Bocchini N, Giantin M, Ferraresso S
Citation(s) 25580480
Submission date Sep 18, 2010
Last update date Dec 21, 2016
Contact name Mery Giantin
E-mail(s) mery.giantin@unipd.it
Phone +39 049 8272935
Organization name University of Padova
Department Dip. Biomedicina Comparata e Alimentazione
Lab Farmacologia e Tossicologia
Street address Viale Università, 16
City Agripolis - Legnaro (PD)
State/province Padova
ZIP/Postal code 35020
Country Italy
 
Platforms (1)
GPL4135 Agilent-014879 Whole Rat Genome Microarray 4x44K G4131F (Feature Number version)
Samples (12)
GSM595528 Rt_h_ctrl_1
GSM595529 Rt_h_ctrl_2
GSM595530 Rt_h_ctrl_3
Relations
BioProject PRJNA132947

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24213_RAW.tar 111.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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