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| Status |
Public on Oct 31, 2010 |
| Title |
The role of integrins in human cytomegalovirus (HCMV)-infected monocytes. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We have established that human cytomegalovirus (HCMV) infection modulates the biology of target primary blood monocytes, allowing HCMV to use monocytes as “vehicles” for its systemic spread. HCMV infection of monocytes results in rapid induction of PI(3)K and NF-κB activity. Integrins, which are upstream of the PI(3)K and NF-κB pathways, were shown to be involved in HCMV binding to and entry into fibroblasts, suggesting that receptor-ligand-mediated signaling following viral binding to integrins on monocytes could trigger the functional changes seen in infected monocytes. We now show that integrin engagement and the activation of the integrin/Src-signaling pathway is essential for the induction of HCMV-infected monocyte motility. To investigate how integrin engagement by HCMV triggers monocyte motility, we examined the infected monocyte transcriptome and found that the integrin/Src-signaling pathway regulates the expression of paxillin, which is an important signal transducer in the regulation of actin rearrangement during cell adhesion and movement. Functionally, we observed that paxillin is activated via the integrin/Src-signaling pathway and is required for monocyte motility. Because motility is intimately connected to cellular cytoskeletal organization, a process that is also important in viral entry, we investigated the role paxillin regulation plays in the process of viral entry of monocytes. New results confirmed that HCMV`s ability to enter target monocytes is significantly inhibited in cells deficient in paxillin expression or that had their integrin/Src/paxillin signaling pathway blocked. From our data, HCMV-cell interactions emerge as an essential trigger for the cellular changes that allow for HCMV entry and hematogenous dissemination.
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| Overall design |
Monocytes were mock-infected, HCMV-infected, or pretreated with PP2 inhibitor prior to HCMV infection. There were three samples analyzed per individual replicate. Three replicates are included.
comparative studies with a use of the specific Src kinase activity inhibitor
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| Contributor(s) |
Nogalski MT, Chan GT, Yurochko AD |
| Citation(s) |
21084488 |
| Submission date |
Sep 20, 2010 |
| Last update date |
Dec 13, 2018 |
| Contact name |
Andrew D. Yurochko |
| E-mail(s) |
ayuroc@lsuhsc.edu
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| Phone |
318-675-8332
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| Fax |
318-675-5764
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| Organization name |
LSUHSC-S
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| Department |
Microbiology & Immunology
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| Street address |
1501 Kings Highway
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| City |
Shreveport |
| State/province |
LA |
| ZIP/Postal code |
71130-3932 |
| Country |
USA |
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| Platforms (1) |
| GPL8300 |
[HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA132939 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24238_RAW.tar |
10.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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