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| Status |
Public on Nov 22, 2010 |
| Title |
Mesenchymal Stem Cells Reduce Inflammation while Enhancing Bacterial Clearance and Improving Survival in Sepsis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Expression data from Total RNA extracted from murine spleen. Sepsis was induced in C57Bl/6J mice by cecal ligation and puncture (CLP), followed 6 hours later by an intravenous injection of Mesenchymal Stem Cell (MSC) or saline. Twenty-eight hours after CLP, plasma, bronchoalveolar lavage (BAL) fluid and tissues were collected for analyses. Total RNA was extracted using Trizol (as per manufactures' instruction) followed by clean-up procedure using Qiagen RNA easy Prep (as per manufactures instructions)
In the following study we hypothesized that mesenchymal stem cells (MSCs), which have documented immunomodulatory properties, would reduce sepsis-associated inflammation and organ injury in a clinically relevant model of sepsis.
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| Overall design |
To identify the molecular changes associated with decreased inflammation in CLP-injured mice treated with MSCs, we analyzed the gene expression profiles from spleens collected at 28 hours from 4 animals per group: sham/saline, CLP/saline, and CLP/MSCs.
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| Contributor(s) |
Mei SH, Haitsma JJ, Dos Santos CC, Deng Y, Lai PF, Slutsky AS, Liles WC, Stewart DJ |
| Citation(s) |
20558630, 33378320 |
| Submission date |
Sep 24, 2010 |
| Last update date |
Jul 14, 2021 |
| Contact name |
Claudia Chimisso dos Santos |
| E-mail(s) |
claudia.santos@utoronto.ca
|
| Organization name |
Saint Michaels Hospital
|
| Department |
University of Toronto
|
| Street address |
30, Bond Street, room 4-011
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5B 1W8 |
| Country |
Canada |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA132813 |
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