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Series GSE24383 Query DataSets for GSE24383
Status Public on Aug 01, 2011
Title The conjunctival transcriptome in scarring trachoma for Tanzanians
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Trachoma is a poorly understood immuno-fibrogenic disease process, initiated by Chlamydia trachomatis (Ct). Differences in conjunctival gene expression profiles between Tanzanians with trachomatous conjunctival scarring (with (TSI) and without (TS) inflammation) and controls (C) were investigated to identify relevant host responses. Tarsal conjunctival swab samples were collected for RNA isolation and Ct PCR. Transcriptome-wide microarray experiments were conducted on 41 samples (TSI 13, TS 15, C14). Cases had enrichment in proinflammatory cytokines and chemokines (IL1B, IL8, CXCL5, CCL20); Matrix metalloprotienases (MMP7, MMP9, MMP12); components of the cornified envelope / squamous metaplasia (SPRR2a, SPRR2F).
 
Overall design Case - control study. Conjunctival swab samples were collected from people with trachomatious conjunctival scarring with (TSI) and without (TS) clinically visable inflammation compared to normal controls (C). Total RNA extracted and analysed on the Illumina WG-6 platrom. Additional extensive qRT-PCR work done on a large set of case-control pairs.
 
Contributor(s) Burton MJ, Hu V, Bauer J, Mabey D, Holland MJ, Bailey RL
Citation(s) 26616738, 22038912
Submission date Sep 27, 2010
Last update date Mar 09, 2020
Contact name Matthew Burton
Organization name London School of Hygiene and Tropical Medicine
Street address Keppel Street
City London
ZIP/Postal code WC1E 7HT
Country United Kingdom
 
Platforms (1)
GPL6884 Illumina HumanWG-6 v3.0 expression beadchip
Samples (42)
GSM601099 Control sample #37
GSM601100 Control sample #32
GSM601101 Trachomatious conjunctival scarring sample #456
Relations
BioProject PRJNA132867

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24383_RAW.tar 6.3 Mb (http)(custom) TAR
GSE24383_non-normalized.txt.gz 13.1 Mb (ftp)(http) TXT
Processed data included within Sample table
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