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Series GSE24469 Query DataSets for GSE24469
Status Public on Dec 22, 2011
Title Cryptochromes mediate rhythmic repression of the glucocorticoid receptor
Organism Mus musculus
Experiment type Expression profiling by array
Summary The circadian transcriptional repressors cryptochromes 1 (Cry1) and 2 (Cry2) interact with the C-terminus of the glucocorticoid receptor (GR) and are required for transrepression in response to the synthetic GR ligand dexamethasone (Dex) in mouse embryonic fibroblasts. Dex induction of many genes was increased in Cry-deficient fibroblasts suggesting that cryptochromes oppose transactivation in addition to contributing to transrepression. In mice, genetic loss of Cry1 and/or Cry2 resulted in glucose intolerance and constitutively high levels of circulating corticosterone, suggesting reduced glucocorticoid suppression of the hypothalamic-pituitary-adrenal axis coupled with increased sensitivity to the hyperglycemic effects of glucocorticoid-mediated transactivation in the liver. Cry1 and Cry2 association with a GRE in the Pck1 promoter was stimulated by Dex, and Dex-induced transcription of pck1 was strikingly increased in Cry-deficient livers. Finally, cry1-/-;cry2-/- mice subjected to 8 weeks of chronic Dex treatment exhibited incomplete suppression of circulating corticosterone and greater glucose intolerance compared with wildtype littermates subjected to the same chronic treatment, consistent with enhanced transcriptional response to the synthetic glucocorticoid ligand.
Overall design Total RNA was obtained from WT and Cry1/2 KO MEFs treated with Dexamethasone (1uM) or control EtOH for 16 hours.
Contributor(s) Yu RT, Lamia KA, Evans RM
Citation(s) 22170608
Submission date Sep 30, 2010
Last update date Jun 14, 2018
Contact name Ruth T Yu
Organization name Salk Institute
Department Gene Expression Lab
Lab Ronald Evans
Street address 10010 N Torrey Pines Rd
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (12)
GSM602717 control EtOH - A
GSM602718 control EtOH - B
GSM602719 control EtOH - C
BioProject PRJNA132767

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Supplementary file Size Download File type/resource
GSE24469_RAW.tar 3.1 Mb (http)(custom) TAR
GSE24469_non-normalized.txt.gz 1.9 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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