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| Status |
Public on May 22, 2011 |
| Title |
Expression profiling of host genes modulated by Epstein-Barr virus (EBV) Rta in HEK293 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
EBV Rta is a transcriptional activator that functions to disrupt EBV latency in cells of epithelial origin. This series of experiment is to identify host genes that are moduated by the expression of doxycycline-inducible EBV Rta in HEK293 cells. Designations for the pooled EBV Rta inducible cell lines is 293TetER; pooled luciferase inducible lines is 293TetLuc (control).
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| Overall design |
Both 293TetER and 293TetLuc cells were grown to log-phase before doxycycline induction. Same number of cells from the two cell lines were treated with doxycycline for 48 h. Experiments were performed in duplicates. Total RNAs from the four samples were extracted and subjected to microarray analysis (Affymetrix Human Gene 1.0 ST Array , n=33,297). Compared to doxycycline treated 293TetLuc, we sought to identify genes up- or down-regulated in by EBV Rta in doxycycline treated 293TetER cells..
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| Contributor(s) |
Lin S, Chen Y, Tsai W, Chen Y, Ko Y |
| Citation(s) |
21423768, 21918011 |
| Submission date |
Oct 07, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Su-Fang Lin |
| E-mail(s) |
sflin1@gmail.com
|
| Organization name |
National Health Research Institutes, Taiwan
|
| Department |
NICR
|
| Lab |
R2-1211
|
| Street address |
No 35, Keyan Road, Zhunan Town
|
| City |
Miaoli Conunty |
| ZIP/Postal code |
35053 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| GSM606063 |
Control, 293TetLuc, doxcycline 48 h, rep1 |
| GSM606064 |
Control, 293TetLuc, doxcycline 48 h, rep2 |
| GSM606065 |
293TetER doxycycline 48 h, rep1 |
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| This SubSeries is part of SuperSeries: |
| GSE24587 |
Epstein–Barr virus (EBV) Rta-mediated cell cycle arrest enables permissive replication of EBV and Kaposi’s sarcoma-associated herpesvirus in 293 cells |
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| Relations |
| BioProject |
PRJNA133583 |
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