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| Status |
Public on Oct 11, 2010 |
| Title |
Expression data from synovium, bone marrow and nucleus pulposus |
| Organisms |
Homo sapiens; Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Synovial and bone marrow mesenchymal stem cells after intradiscally injection show regenerative effects of nucleus pulposus.
Microarray analyses of rats were performed to investigate the closeness of the gene profiles between the nucleus pulposus cells and the synovial or bone marrow mesenchymal stem cells. To investigate interaction between synovial mesenchymal stem cells and nucleus pulposus cells, human synovial mesenchymal stem cells and rat nucleus pulposus cells were co-cultured, and species specific microarray were performed.
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| Overall design |
Synovium of knee joints, bone marrow and nucleus pulposus were harvested from rat or human, and cells were isolated for RNA extraction and hybridization on Affymetrix microarrays. To compare the gene profiles each other, isolated cells were mono-cultured respectively, and human synovial mesenchymal stem cells and rat nucleus pulposus cells were co-cultured.
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| Contributor(s) |
Miyamoto T, Sekiya I, Matsumoto K |
| Citation(s) |
21054867 |
| Submission date |
Oct 11, 2010 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Takashi Miyamoto |
| E-mail(s) |
miyamoto.orj@tmd.ac.jp
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| Organization name |
Tokyo Medical and Dental University
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| Department |
Department of Orthopedic Surgery
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| Street address |
1-5-45 Yushima, Bunkyo-ku
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| City |
Tokyo |
| ZIP/Postal code |
113-8519 |
| Country |
Japan |
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| Platforms (2) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (10)
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| GSM606836 |
rat synovial mesenchymal stem cell, mono-culture, rep1 |
| GSM606837 |
rat bone marrow mesenchymal stem cell, mono-culture, rep1 |
| GSM606838 |
rat nucleus pulposus cell, mono-culture, rep1 |
| GSM606839 |
rat nucleus pulposus cell, mono-culture, rep2 |
| GSM606840 |
rat nucleus pulposus cell, co-culture, rep1 |
| GSM606841 |
rat nucleus pulposus cell, co-culture, rep2 |
| GSM606843 |
human synovial mensenchymal stem cell, mono-culture, rep1 |
| GSM606844 |
human synovial mensenchymal stem cell, mono-culture, rep2 |
| GSM606845 |
human synovial mensenchymal stem cell, co-culture, rep1 |
| GSM606846 |
human synovial mensenchymal stem cell, co-culture, rep2 |
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| Relations |
| BioProject |
PRJNA132551 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24612_RAW.tar |
56.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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