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| Status |
Public on Aug 11, 2011 |
| Title |
Image defocus and altered retinal gene expression in chicks |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by array
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| Summary |
Because refractive development is governed largely by the retina, we analyzed the retinal transcriptome in chicks wearing a spectacle lens, a well-established means to induce refractive errors, to identify gene expression alterations and to develop novel mechanistic hypotheses about refractive development.
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| Overall design |
One-week-old white Leghorn chicks wore a unilateral spectacle lens of + or –15 diopters for 6 hours or 3 days (n=6 for each condition). With total RNA from the retina/retinal pigment epithelium (RPE), Affymetrix Chicken GeneChips were used to compare gene expression levels between lens-wearing and contralateral control eyes. Normalized microarray signal intensities were evaluated by an analysis of variance approach. Selected differentially expressed genes were validated by qPCR in biologically independent samples.
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| Contributor(s) |
Stone RA, McGlinn AM, Baldwin DA, Tobias JW, Iuvone PM, Khurana TS |
| Citation(s) |
21642623 |
| Submission date |
Oct 12, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Alice M McGlinn |
| E-mail(s) |
mcglinn@mail.med.upenn.edu
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| Phone |
215-898-6508
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| Fax |
215-898-0528
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| Organization name |
University of Pennsylvania School of Medicine
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| Department |
Ophthalmology
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| Lab |
Dr Richard Stone
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| Street address |
602D Richards Building 3700 Hamilton Walk
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104-6075 |
| Country |
USA |
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| Platforms (1) |
| GPL3213 |
[Chicken] Affymetrix Chicken Genome Array |
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| Samples (48)
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| GSM607659 |
chicken eye, control, 6 hours, biological replicate 1_Chicken_396c |
| GSM607660 |
chicken eye, -15 diopter lens, 6 hours,biological replicate 1_Chicken_396e |
| GSM607661 |
chicken eye, control, 6 hours, biological replicate 2_Chicken_397c |
| GSM607662 |
chicken eye, -15 diopter lens, 6 hours,biological replicate 2_Chicken_397e |
| GSM607663 |
chicken eye, control, 6 hours, biological replicate 3_Chicken_398c |
| GSM607664 |
chicken eye, -15 diopter lens, 6 hours, biological replicate 3_Chicken_398e |
| GSM607665 |
chicken eye, control, 6 hours, biological replicate 4_Chicken_399c |
| GSM607666 |
chicken eye, -15 diopter lens, 6 hours, biological replicate 4_Chicken_399e |
| GSM607667 |
chicken eye, control, 6 hours, biological replicate 5_Chicken_400c |
| GSM607668 |
chicken eye, -15 diopter lens, 6 hours,biological replicate 5_Chicken_400e |
| GSM607669 |
chicken eye, control, 6 hours, biological replicate 6_Chicken_401c |
| GSM607670 |
chicken eye, -15 diopter lens, 6 hours, biological replicate 6_Chicken_401e |
| GSM607671 |
chicken eye, control, 6 hours, biological replicate 1_Chicken_421c |
| GSM607672 |
chicken eye, +15 diopter lens, 6 hours, biological replicate 1_Chicken_421e |
| GSM607673 |
chicken eye, control, 6 hours, biological replicate 2_Chicken_422c |
| GSM607674 |
chicken eye, +15 diopter lens, 6 hours, biological replicate 2_Chicken_422e |
| GSM607675 |
chicken eye, control, 6 hours, biological replicate 3_Chicken_423c |
| GSM607676 |
chicken eye, +15 diopter lens, 6 hours, biological replicate 3_Chicken_423e |
| GSM607677 |
chicken eye, control, 6 hours, biological replicate 4_Chicken_424c |
| GSM607678 |
chicken eye, +15 diopter lens, 6 hours, biological replicate 4_Chicken_424e |
| GSM607679 |
chicken eye, control, 6 hours, biological replicate 5_Chicken_425c |
| GSM607680 |
chicken eye, +15 diopter lens, 6 hours, biological replicate 5_Chicken_425e |
| GSM607681 |
chicken eye, control, 6 hours, biological replicate 6_Chicken_426c |
| GSM607682 |
chicken eye, +15 diopter lens, 6 hours, biological replicate 6_Chicken_426e |
| GSM607683 |
chicken eye, control, 3 days, biological replicate 1_Chicken_406c |
| GSM607684 |
chicken eye, -15 diopter lens, 3 days, biological replicate 1_Chicken_406e |
| GSM607685 |
chicken eye, control, 3 days, biological replicate 2_Chicken_407c |
| GSM607686 |
chicken eye, -15 diopter lens, 3 days, biological replicate 2_Chicken_407e |
| GSM607687 |
chicken eye, control, 3 days, biological replicate 3_Chicken_408c |
| GSM607688 |
chicken eye, -15 diopter lens, 3 days, biological replicate 3_Chicken_408e |
| GSM607689 |
chicken eye, control, 3 days, biological replicate 4_Chicken_409c |
| GSM607690 |
chicken eye, -15 diopter lens, 3 days, biological replicate 4_Chicken_409e |
| GSM607691 |
chicken eye, control, 3 days, biological replicate 5_Chicken_410c |
| GSM607692 |
chicken eye, -15 diopter lens, 3 days, biological replicate 5_Chicken_410e |
| GSM607693 |
chicken eye, control, 3 days, biological replicate 6_Chicken_411c |
| GSM607694 |
chicken eye, -15 diopter lens, 3 days, biological replicate 6_Chicken_411e |
| GSM607695 |
chicken eye, control, 3 days, biological replicate 1_Chicken_433c |
| GSM607696 |
chicken eye, +15 diopter lens, 3 days, biological replicate 1_Chicken_433e |
| GSM607697 |
chicken eye, control, 3 days, biological replicate 2_Chicken_434c |
| GSM607698 |
chicken eye, +15 diopter lens, 3 days, biological replicate 2_Chicken_434e |
| GSM607699 |
chicken eye, control, 3 days, biological replicate 3_Chicken_435c |
| GSM607700 |
chicken eye, +15 diopter lens, 3 days, biological replicate 3_Chicken_435e |
| GSM607701 |
chicken eye, control, 3 days, biological replicate 4_Chicken_436c |
| GSM607702 |
chicken eye, +15 diopter lens, 3 days, biological replicate 4_Chicken_436e |
| GSM607703 |
chicken eye, control, 3 days, biological replicate 5_Chicken_437c |
| GSM607704 |
chicken eye, +15 diopter lens, 3 days, biological replicate 5_Chicken_437e |
| GSM607705 |
chicken eye, control, 3 days, biological replicate 6_Chicken_438c |
| GSM607706 |
chicken eye, +15 diopter lens, 3 days, biological replicate 6_Chicken_438e |
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| Relations |
| BioProject |
PRJNA132531 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24641_RAW.tar |
182.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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