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Series GSE24776 Query DataSets for GSE24776
Status Public on Mar 02, 2012
Title Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response (WT and G9a deficient MEFs)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Effective anti-viral immunity depends on the ability of infected cells or cells triggered with virus-derived nucleic acids to produce type I interferon (IFN), which activates transcription of numerous antiviral genes. However, disproportionately strong or chronic IFN expression is a common cause of inflammatory and autoimmune diseases. Here we describe an epigenetic mechanism that determines cell-type specific differences in IFN and IFN-stimulated gene expression in response to exogenous signals. We identify di-methylation of histone H3 at lysine 9 (H3K9me2) as a suppressor of IFN and IFN-inducible antiviral gene expression. We show that levels of H3K9me2 at IFN and IFN stimulated genes (ISG) correlate inversely with the scope and amplitude of IFN and ISG expression in fibroblasts and dendritic cells. Accordingly, genetic ablation or pharmacological inactivation of lysine methyltransferase G9a, which is essential for the generation of H3K9me2, resulted in phenotypic conversion of fibroblasts into highly potent IFN-producing cells and rendered these cells resistant to pathogenic RNA viruses. In summary, our studies implicate H3K9me2 and enzymes controlling its abundance as key regulators of innate antiviral immunity.
 
Overall design Examination of gene expression in response to PolyI:C in WT and G9a deficient MEFs
 
Contributor(s) Fang TC, Tarakhovsky A
Citation(s) 22412156
Submission date Oct 18, 2010
Last update date Jun 14, 2018
Contact name Terry Fang
E-mail(s) tfang@rockefeller.edu
Phone 212-327-8265
Fax 212-327-8258
Organization name Rockefeller University
Street address 1230 York Ave
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (26)
GSM610302 G9a f/f 0h pIC Rep1
GSM610303 G9a f/f 0h pIC Rep2
GSM610304 G9a f/f 2h pIC Rep1
This SubSeries is part of SuperSeries:
GSE24826 Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response
Relations
BioProject PRJNA133385

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24776_RAW.tar 3.1 Mb (http)(custom) TAR
GSE24776_non-normalized.txt.gz 4.1 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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