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| Status |
Public on Feb 22, 2011 |
| Title |
Neonatal beta cells lack the specialized metabolic phenotype of mature beta cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Fetal and neonatal beta cells have poor glucose-induced insulin secretion and only gain robust glucose responsiveness several weeks after birth. This unresponsiveness may be due to a generalized immaturity of the metabolic pathways normally found in beta cells.
Gene expression profile of neonatal (1 day old) and young adult (6 weeks old) Sprague-Dawley rat islets were evaluated and compared.
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| Overall design |
Frozen sections were obtained from pancreases of neonatal (1 day old) and young adult (6 weeks old) Sprague-Dawley rats. The beta cell enriched cores of the islets were excised using laser capture microdissection. RNA was extracted, amplified and subjected to microarray analysis.
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| Contributor(s) |
Jermendy A, Toschi E, Aye T, Koh A, Aguayo-Mazzucato C, Sharma A, Weir GC, Sgroi DC, Bonner-Weir S |
| Citation(s) |
21240476 |
| Submission date |
Oct 19, 2010 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Susan Bonner-Weir PhD |
| E-mail(s) |
susan.bonner-weir@joslin.harvard.edu
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| Organization name |
Joslin Diabetes Center
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| Department |
Section on Islet Cell and Regenerative Biology
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| Street address |
One Joslin Place
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL85 |
[RG_U34A] Affymetrix Rat Genome U34 Array |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA132245 |
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