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| Status |
Public on Oct 19, 2011 |
| Title |
Lack of hepatic response of microRNA in mice following chronic benzo(a)pyrene exposure (gene expression) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Benzo[a]pyrene (BaP) is a very extensively studied prototypical polycyclic aromatic hydrocarbons (PAHs). Previous work in our laboratory showed no changes of microRNA (miRNA) expression in liver following a 3 days exposure to BaP, suggesting a lack of miRNA transcriptional responses to aryl hydrocarbon receptor agonists and/or DNA damage. Here, we studied 25-week old male MutaTM Mouse exposed to 25, 50, and 75 mg/kg/day BaP by oral gavage for 28 consecutive days. MAANOVA identified 110 differentially expressed genes (adjusted p < 0.05) with fold change greater than 1.5. The genes most affected included those involved in xenobiotic metabolism, phase II metabolizing enzymes, as well as the downstream targets of p53. Pathway specific RT-PCR was used to confirm the p53 response. A single significant increase in miRNA expression was identified (mir-34a and validated using the Qiagen miScript PCR system). This miRNA is known to be transcriptionally activated by p53. Ingestion of BaP leads to widespread changes in gene expression in mouse liver, with enrichment of genes involved in cell cycle arrest, DNA damage response, and apoptosis via the p53 pathway. In contrast, miRNA expression was relatively unaffected. Only miR-34a was significantly up-regulated, and may therefore play a critical role in the post-transcriptional regulation of p53 and/or its downstream targets.
Keywords: Agilent mouse 4 x 44 oligonucleotide microarrays were used to assess global gene expression in response to 25, 50, and 75 mg/kg BaP treatment
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| Overall design |
Individual total RNA (200 ng) from 5 mice per treatment group (control, 24mg/kg, 50mg/kg, and 75mg/kg) and universal reference total RNA (Stratagene, Mississauga, ON, Canada) was used to synthesize double stranded cDNA.
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| Contributor(s) |
Malik AI, Williams A, Lemieux CL, White PA, Yauk CL |
| Citation(s) |
21964900 |
| Submission date |
Oct 25, 2010 |
| Last update date |
May 10, 2018 |
| Contact name |
Andrew Williams |
| Organization name |
Health Canada
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| Street address |
50 Columbine
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| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1A OK9 |
| Country |
Canada |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (20)
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| This SubSeries is part of SuperSeries: |
| GSE24910 |
Lack of hepatic response of microRNA in mice following chronic benzo(a)pyrene exposure |
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| Relations |
| BioProject |
PRJNA133399 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24907_RAW.tar |
87.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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