|
| Status |
Public on Oct 10, 2011 |
| Title |
MicroRNA-145 Regulates Human Corneal Epithelial Differentiation [Agilent-014850 array data] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To investigate the gene expression in human corneal epithelial overexpressing hsa-miR-145 by transfection , we have employed Whole Human Genome Oligo Microarray (Agilent) as a screening platform to identify gene regulation.
We discovered a differential gene expression in HCE cells transfected with hsa-mIR-145 against cells with scrambled sequences. Among them, genes related with corneal development, integrity, differentiation and inflammatory responses were found and this was validated by real-time PCR.
|
| |
|
| Overall design |
HCE cells transfected with hsa-miR-145 or scrambled sequences were collected at 24 hours after transfection. Total RNA was extracted by Trizol/chloroform and purified with RNeasy mini spin column. RNA samples with 28S/18S ratios in the range of 1.4 to 1.8 were used for expression RNA profiling using Whole Human Genome Oligo Microarray (Agilent).
|
| |
|
| Contributor(s) |
Pang C, Yam GH |
| Citation(s) |
21701675 |
| Submission date |
Oct 28, 2010 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Gary Yam |
| E-mail(s) |
gary_yam@cuhk.edu.hk
|
| Organization name |
Chinese University of Hong Kong
|
| Street address |
147K Argyle St, Mongkok
|
| City |
Hong Kong |
| ZIP/Postal code |
NA |
| Country |
China |
| |
|
| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
|
| Samples (2) |
|
| This SubSeries is part of SuperSeries: |
| GSE24981 |
MicroRNA-145 Regulates Human Corneal Epithelial Differentiation |
|
| Relations |
| BioProject |
PRJNA133463 |
