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Series GSE24993 Query DataSets for GSE24993
Status Public on May 29, 2011
Title Expression Profiling of Tissues versus Primary Cultures from Colorectal Cancer Patients
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The main aim of this study is to evaluate how comparable the established colorectal primary tumor cultures are to the original tumor tissues obtained directly from patients in terms of their genomic profile for which they can be eventually utilized to improve in vitro drug assessment and facilitate personalized treatment. Here, we used colorectal cancer patient samples collected after surgery to perform a gene expression comparison study between original tissues and self-established primary cultures. Subsequently, we also investigated the possibility of using primary cells from patients’ tumors as a model for assessing drug response by characterizing the modifications in gene-drug associations of the primary cells which occurred during the establishment of cell cultures. On an auxiliary note, cytogenetic studies are only possible in cultured cells and not in tissue samples, hence, in this study we also evaluated the potential of genome stability analysis on primary cells as an alternative method to improve our understanding of the genomic changes which occur in cancer initiation and progression.
 
Overall design Using cRNA microarrays, gene expression of low-passage colorectal tumor primary cultures were compared against colorectal tumor tissues obtained directly from patients.
 
Contributor(s) Bellot GL, Wong J, Tan WH, Tay LL, Koh D, Wang X
Citation(s) 22186935
Submission date Oct 28, 2010
Last update date Jan 18, 2013
Contact name Sherry Wang
E-mail(s) bchdm@nus.edu.sg
Organization name National University of Singapore
Department Biochemistry
Street address 21 Lower Kent Ridge Road
City Singapore
ZIP/Postal code 119077
Country Singapore
 
Platforms (2)
GPL6104 Illumina humanRef-8 v2.0 expression beadchip
GPL14186 Illumina HumanRef-8 v2 Expression Beadchip 1699538181
Samples (17)
GSM613933 NT09/323 Normal_Colon (1699538179_B)
GSM613934 NT09/350 Tumor_Colon (1699538179_C)
GSM613935 NT09/340 Tumor_Colon (1699538179_D)
Relations
BioProject PRJNA131973

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24993_RAW.tar 3.4 Mb (http)(custom) TAR
GSE24993_non-normalized.txt.gz 4.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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