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| Status |
Public on Dec 21, 2010 |
| Title |
Transcriptional response to serum stimulation of Rat1 fibroblasts in the presence or absence of the Myc interfering molecule termed Omomyc. |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Myc is a multifaceted bHLHZip transcription factor deregulated in the majority of human cancers. How to target Myc for cancer therapy is unclear, given its involvement in a variety of key functions in healthy cells. We used microarrays to capture the cellular transcriptional response to Omomyc – a Myc interfering molecule acting at the level of protein protein interactions that demonstrated a remarkable therapeutic efficacy in transgenic mouse cancer models.
We found that Omomyc differently affects Myc induced gene repression and activation, channelling the Myc interactome activity to repression.
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| Overall design |
To determine whether Omomyc causes widespread changes of the cell transcriptome, we analysed the transcriptional response to serum stimulation of Rat1 fibroblasts stably infected with an Omomer – Omomyc fused to the tamoxifen inducible oestrogen receptor ERTM – producing retrovirus (Rat1_Omomer) as compared to Rat1 cells infected with a control virus (Rat1_Control). Endogenous c-Myc is known to be sharply induced by serum and is critical for cell cycle re-entry. Cells were grown, serum-starved for 48 h in presence of tamoxifen (4-OHT) and stimulated by addition of fresh serum. Total RNA was collected from Rat1_Control and Rat1-Omomer cells at the time of serum re-addition (T0) and 90' thereafter (T90'), in the presence of tamoxifen.
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| Contributor(s) |
Nasi S, Savino M, Soucek L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 29, 2010 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Sergio Nasi |
| E-mail(s) |
sergio.nasi@uniroma1.it
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| Phone |
0649912241
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| Fax |
0649912500
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| Organization name |
CNR - Consiglio Nazionale delle Ricerche
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| Department |
IBPM - Sapienza Università di Roma
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| Street address |
P.le A. Moro 5
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| City |
Roma |
| State/province |
Italia |
| ZIP/Postal code |
00185 |
| Country |
Italy |
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| Platforms (1) |
| GPL85 |
[RG_U34A] Affymetrix Rat Genome U34 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA134775 |
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