|
| Status |
Public on Aug 01, 2021 |
| Title |
Analysis of mouse central bone marrow versus endosteal transcriptome to define the hematopoietic stem cell niche at the endosteum |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Stem cell function is regulated by specialized microenvironments called stem cell niches. These niches maintain stem cells in a dormant state and promote self-renewal. The most potent hematopoietic stem cells (HSC) with high self-renewal potential are reportedly enriched in the endosteal compared to the central region of the bone marrow. Therefore we analyzed the global transcriptome of the endosteal region and directly compared it to that of the central bone marrow (BM). This comparative, differential analysis revealed that in addition to genes specific to the osteoblastic and osteoclastic lineage and classic regulators of HSC (CXCL12, KIT ligand, angiopoietin-1, Jagged-1, N-cadherin), the endosteum abundantly expresses prostaglandin I2 (PGI2) synthase (Ptgis), which produces PGI2. PGI2 is a highly labile, lipid metabolite with no known roles in regulating HSCs. We show in this study that PGI2 is a potent regulator of HSC function. Therefore comparing endosteal versus central BM transcriptome is a viable approach for uncovering candidate genes that may regulate the function of HSC and the HSC niche.
|
| |
|
| Overall design |
Femurs from 3 individual C57BL/6 mice were collected and RNA expression profiles from the central bone marrow and endosteal regions compared. Affymetrix IDs were compared by calculating the log2 of the average endosteal bone marrow signal divided by the average central bone marrow signal.
|
| |
|
| Contributor(s) |
Tay J, Barbier V, Helwani FM, Price GR, Levesque J, Winkler IG |
| Citation(s) |
34260805 |
| Submission date |
Nov 01, 2010 |
| Last update date |
Nov 01, 2021 |
| Contact name |
Gareth Price |
| E-mail(s) |
nscalehome@gmail.com
|
| Phone |
61413289611
|
| Organization name |
Queensland Facility for Advanced Bioinformatics
|
| Street address |
Carmody Road
|
| City |
Brisbane |
| State/province |
Queensland |
| ZIP/Postal code |
4072 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA134747 |
Less...
More...