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Series GSE25156 Query DataSets for GSE25156
Status Public on May 01, 2011
Title Host Factors with Reduced Expression in Two HCV Permissive Cell Lines as Compared to the Non-Permissive Parent Cell Line Huh7
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Drugs directly targeting Hepatitis C (HCV) are often rendered useless by the high mutation rate of the virus. Thus, we deduce that targeting of host factor that affect HCV replication may provide enhanced therapy fort HCV infection. Hepatocyte cell line Huh7 is known to be non-permissive for Hepatits C (HCV) replication. Through a method developed by the Rice laboratory (Blight, K.J., et al., J Virol, 2002), selection of a small subset of permissive hepatocytes is possible. The Rice laboratory generated the first permissive cell line, Huh7.5, using this method. We generated another permissive cell line, HRP1, using the same method.
With microarray, we compared the expression of host mRNAs in non-permissive Huh7 to both Huh7.5 and HRP1 searching for host factors lost in the cell lines permisive for HCV replication.
 
Overall design Non-permissive cell line Huh7 and permissive cell lines Huh7.5 and HRP1 were harvested for RNA extraction and hybridization on Affymetrix microarrays.
 
Contributor(s) Chen Q, Abdalla S, Ye J
Citation(s) 21767813
Submission date Nov 05, 2010
Last update date Mar 25, 2019
Contact name Bray Denard
Organization name University of Texas Southwestern Medical Center
Department Molecular Genetics
Lab Jin Ye Lab
Street address 5323 Harry Hines Blvd. L5.226
City Dallas
State/province TX
ZIP/Postal code 75390
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (4)
GSM618131 HUH7
GSM618132 HRP1
GSM618133 #2691 HUH7
Relations
BioProject PRJNA134343

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25156_RAW.tar 90.9 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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