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Series GSE25211 Query DataSets for GSE25211
Status Public on Nov 12, 2010
Title IL-1β-driven neutrophil biogenesis and survival preserve anti-bacterial defense in IKKβ deficiency
Organism Mus musculus
Experiment type Expression profiling by array
Summary IKKβ-dependent NF-κB activation is associated with inflammation and tumorigenesis and is critical for innate immunity. Despite risk of immune suppression, pharmacological blockade of IKKβ/NF-κB has been considered as an attractive strategy for treatment of inflammatory diseases and cancer. Unexpectedly, treatment with IKKβ inhibitors caused neutrophilia, which also occurs in mice with inducible IKKβ deletion, termed IkkβΔ mice. IkkβΔ mice show hyperproliferation of granulocyte progenitors and increased neutrophil lifespan. Deletion of IL-1 receptor 1 in IkkβΔ mice restored normal blood cellularity and prevented neutrophil-driven inflammation. However, IkkβΔ/Il-1r1-/- mice, in contrast to IkkβΔ mice, are highly susceptible to bacterial infections indicating that either IKKβ/NF-κB or IL-1R signaling are sufficient for maintaining anti-microbial defenses, but inactivation of both pathways severely compromises innate immunity.
 
Overall design Thioglycollate-elicited peritoneal neutrophils were purified from IkkβF/F and IkkβΔ mice using anti-Ly-6G magnetic beads and cultured. Ly-6G+ neutrophils were collected and total RNA was extracted with TRIzol® (Invitrogen) and was purified by RNA micro cleanup Kit (Qiagen). Biotinylated cRNA was synthesized using an RNA Amplification Kit (Ambion) following manufacturer's directions. Biotin-labeled cRNA was hybridized to Illumina Mouse-Ref8 BeadChips (Illumina) and the results were analyzed using BeadStudio v3.1 software. Data analysis and quality control were carried out using Partek® software.
 
Contributor(s) Hsu L, Enzler T, Seita J, Timmer AM, Lee C, Lai T, Yu G, Lai L, Temkin V, Nizet V, Weissman IL, Karin M
Citation(s) 21170027
Submission date Nov 09, 2010
Last update date Jun 14, 2018
Contact name Liang-Chuan Lai
E-mail(s) llai@ntu.edu.tw
Phone 886-2-23123456
Organization name National Taiwan University
Department Physiology
Street address 1 Jen-Ai Road, Sec 1
City Taipei
State/province Taiwan
ZIP/Postal code 10051
Country Taiwan
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (4)
GSM619274 IkkβΔ_replicate1
GSM619275 IkkβΔ_replicate2
GSM619276 IkkβF/F_replicate1
Relations
BioProject PRJNA134329

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25211_RAW.tar 3.1 Mb (http)(custom) TAR
GSE25211_non-normalized_data.txt.gz 1.4 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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