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Series GSE2527 Query DataSets for GSE2527
Status Public on May 06, 2005
Title Gata-1 Knock Down - Wild Type Megakaryocyte Gene Expression
Organism Mus musculus
Experiment type Expression profiling by array
Summary Hematopoietic progenitor cells were isolated from 13.5 day mouse fetal livers by lineage depletion and expanded for three days. Fetal livers were isolated from both wild type and Gata-1 knock embryos. Gata-1 knock embryos contain a deletion of the Gata-1 promoter sequence that results in undetectable levels of Gata-1 protein specifically in the megakaryocyte lineage. Following progenitor outgrowth megakaryocytes were enriched in a differentiation media for three days and isolated on a discontinuous BSA gradient. The resulting megakaryocytes were >90% pure as determined by acetylcholinesterase staining. These cells were lysed in Trizol and the resulting RNA was used for hybridization.
Keywords = Gata-1
Keywords = Megakaryocytes
Keywords: other
Contributor(s) Muntean AG, Crispino JD
Citation(s) 15860665
Submission date Apr 14, 2005
Last update date Feb 11, 2019
Contact name John Crispino
Phone (773) 834-7457
Organization name The University of Chicago
Street address
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM48230 Gata-1 Knock Down A
GSM48231 Gata-1 Knock Down B
GSM48232 Gata-1 Knock Down C
BioProject PRJNA92071

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