|
| Status |
Public on Dec 10, 2010 |
| Title |
Altered antisense-to-sense transcript ratios in breast cancer: Sage-Seq |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Transcriptome profiling studies suggest that a large fraction of the genome is transcribed and many transcripts function independent of their protein coding potential. The relevance of noncoding RNAs (ncRNAs) in normal physiological processes and in tumorigenesis is increasingly recognized. Here, we describe consistent and significant differences in the distribution of sense and antisense transcripts between normal and neoplastic breast tissues. Many of the differentially expressed antisense transcripts likely represent long ncRNAs. A subset of genes that mainly generate antisense transcripts in normal but not cancer cells is involved in essential metabolic processes. These findings suggest fundamental differences in global RNA regulation between normal and cancer cells that might play a role in tumorigenesis.
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| Overall design |
Global transcriptome profilings of 12 samples in normal and 9 samples in cancer from clinical breast tissue using Sage-Seq.
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| |
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| Contributor(s) |
Polyak K, Reo M, Shipitsin M, Choudhury S, Wu ZJ, Protopopov A, Yao J, Lo P, Bessarabova M, Ishkin A, Nikolsky Y, Liu XS, Sukumar S |
| Citation(s) |
21098291 |
| Submission date |
Nov 11, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Kornelia Polyak |
| E-mail(s) |
kornelia_polyak@dfci.harvard.edu
|
| Phone |
617-632-2106
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Medical Oncology
|
| Lab |
Polyak
|
| Street address |
450 Brookline Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
|
| Samples (21)
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| This SubSeries is part of SuperSeries: |
| GSE25292 |
Altered antisense-to-sense transcript ratios in breast cancer |
|
| Relations |
| SRA |
SRP004847 |
| BioProject |
PRJNA142565 |
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