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Series GSE25337 Query DataSets for GSE25337
Status Public on Jan 12, 2011
Title Gene expression data of Sox17-GFP(+) mouse definitive endoderm before and after expansion on mesenchyme.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mouse embryonic stem cells containing a Sox17-GFP construct were differentiated using growth factors (Activin A and Wnt3A) to definitive endoderm. Sox17-GFP(+) cells were sorted using fluorescence activated cell sorting and either used for total RNA harvest OR continued in culture in the presence of primary pancreatic mesenchymal cell lines. At the end of 6 serial passages on mesenchyme, the Sox17-GFP(+) cells were again sorted and the RNA was harvested for arrays.
 
Overall design Samples were prepared as described in summary, with technical duplicates for each of the following 3 categories: 1. Unpassaged (P0) endoderm, 2. Endoderm passaged 6 times (P6) on mesenchyme 1, and 3. Endoderm passaged 6 times (P6) on mesenchyme 2.
 
Contributor(s) Sneddon JB, Borowiak M, Melton DA
Citation(s) 23041930
Submission date Nov 15, 2010
Last update date May 21, 2019
Contact name Julie Beth Sneddon
Organization name Harvard University
Department Department of Stem Cells and Regenerative Biology
Lab Douglas Melton Lab
Street address 7 Divinity Avenue
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (6)
GSM623458 Endoderm_unpassaged_rep1
GSM623459 Endoderm_unpassaged_rep2
GSM623460 Endoderm_passage6_mes1_rep1
Relations
BioProject PRJNA134867

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25337_RAW.tar 3.1 Mb (http)(custom) TAR
GSE25337_non-normalized.txt.gz 1.0 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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