|
| Status |
Public on Jul 01, 2011 |
| Title |
Liver-expressed antimicrobial peptide-2 is downregulated in Eimeria maxima-infected chickens |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by array
|
| Summary |
Avian coccidiosis is a major disease of poultry caused by the intestinal protozoa Eimeria. Aviagen line A and line B birds show differential susceptibility to Eimeria infection, with line B birds exhibiting higher lesion scores and mortality. The objective of this study was to examine differential intestinal gene expression between line A and B chicks in response to a challenge with Eimeria maxima. Following challenge with 1 x 10^4 oocysts/chick, greater than 40% of line A chicks had lesion scores of 0 to 1 (on 0 to 4 scale), similar to controls. In contrast, all line B challenged chicks at this same dose had lesion scores of 2 to 4.
|
| |
|
| Overall design |
Total RNA was extracted from the jejunum of control and challenged chicks from both lines A and B. Microarrays were used for detecting the expression-changed genes which responded to the Eimeria challenge.
Samples included: four control A chicks, two challenged A chicks with a lesion score of 1 (A/LS1), two challenged A chicks with lesion scores of 3 to 4 (A/LS3-4), four control B chicks, two challenged B chicks with lesion scores of 2 to 3 (B/LS2-3) and two challenged B chicks with lesion scores of 4 (B/LS4). The DNA microarrays were processed at the Virginia Bioinformatics Institute (Virginia Tech) core facility. The raw array data were normalized using GC-robust multiple array (GC-RMA) normalization. Analysis of variance was used for differentially expressed genes based on Welch ANOVA (P < 0.05) and probe set lists were ordered using the fold change analysis provided by GeneSpring software.
|
| |
|
| Contributor(s) |
Casterlow S, Li H, Gilbert ER, Dalloul RA, McElroy AP, Emmerson DA, Wong EA |
| Citation(s) |
21597061 |
| Submission date |
Nov 23, 2010 |
| Last update date |
Mar 30, 2012 |
| Contact name |
Huifeng Li |
| E-mail(s) |
lihuifengtom@yahoo.com.cn
|
| Phone |
1-540-231-3183
|
| Fax |
1-540-231-3010
|
| Organization name |
Virginia Tech
|
| Department |
Animal and Poultry Sciences
|
| Lab |
Biotechnology
|
| Street address |
3570 Litton Reaves Hall
|
| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24061-0306 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL3213 |
[Chicken] Affymetrix Chicken Genome Array |
|
| Samples (16)
|
| GSM629081 |
Line A chicken intestine control 1 with Lesion Score 0 |
| GSM629082 |
Line A chicken intestine control 2 with Lesion Score 0 |
| GSM629083 |
Line A chicken intestine control 3 with Lesion Score 0 |
| GSM629084 |
Line A chicken intestine control 4 with Lesion Score 1 |
| GSM629085 |
Line A chicken intestine challenged 1 with Lesion Score 1 |
| GSM629086 |
Line A chicken intestine challenged 2 with Lesion Score 1 |
| GSM629087 |
Line A chicken intestine challenged 3 with Lesion Score 3 |
| GSM629088 |
Line A chicken intestine challenged 4 with Lesion Score 4 |
| GSM629089 |
Line B chicken intestine control 1 with Lesion Score 0 |
| GSM629090 |
Line B chicken intestine control 2 with Lesion Score 0 |
| GSM629091 |
Line B chicken intestine control 3 with Lesion Score 0 |
| GSM629092 |
Line B chicken intestine control 4 with Lesion Score 1 |
| GSM629093 |
Line B chicken intestine challenged 1 with Lesion Score 2 |
| GSM629094 |
Line B chicken intestine challenged 2 with Lesion Score 3 |
| GSM629095 |
Line B chicken intestine challenged 3 with Lesion Score 4 |
| GSM629096 |
Line B chicken intestine challenged 4 with Lesion Score 4 |
|
| Relations |
| BioProject |
PRJNA134225 |
Less...
More...