|
| Status |
Public on Dec 01, 2010 |
| Title |
Expression Profiling: during in vitro neural differentiation from mES cells. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
GeneChip-based screen for genes induced in the initial phase of neural differentiation from ES cells.
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| Overall design |
We established an efficient system for the in vitro neural differentiation of mouse ES cells involving floating aggregate culture in growth-factor-minimized medium (serum-free culture of embryoid body-like aggregates; SFEB, http://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/pubmed/15696161). We use this culture system, Sox1-GFP ES cells (http://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/pubmed/12923295) were differentiated with SFEB culture method for 1-5 days. Then, differentiated cells were sorted with Sox1 (neuroectodermal marker) or E-cadherin (undifferentiation marker) by FACS. And analyzed these cell populations by using Affymetrix Murine Genome U74 Version 2 Arrays (U74Av2, U74Bv2 and U74Cv2).
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| Contributor(s) |
Kamiya D, Jakt LM, Okada M |
| Citation(s) |
21326203 |
| Submission date |
Nov 24, 2010 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Daisuke Kamiya |
| E-mail(s) |
kamiya@cdb.riken.jp
|
| Organization name |
RIKEN CDB
|
| Street address |
2-2-3 Minatojima-Minamimachi, Chuo
|
| City |
Kobe |
| ZIP/Postal code |
6500047 |
| Country |
Japan |
| |
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| Platforms (3) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
| GPL82 |
[MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array |
| GPL83 |
[MG_U74Cv2] Affymetrix Murine Genome U74 Version 2 Array |
|
| Samples (30)
|
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| Relations |
| BioProject |
PRJNA134235 |
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