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| Status |
Public on Nov 01, 2013 |
| Title |
Transcriptional programs controlled by NGF signaling in developing mouse dorsal root ganglia neurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
During embryogenesis, nociceptive sensory neurons of the dorsal root ganglia depend intimately on Nerve Growth Factor (NGF) for neuronal survival, maturation and target innervation. NGF is a secreted molecular signal synthesized by neuronal target tissues. In developing nociceptors, NGF engages the receptor tyrosine kinase TrkA to activate a gene transcriptional program involving the regulation of hundreds of transcripts. To identify NGF-dependent genes in developing mouse nociceptors, we have designed and performed two separate microarray screens to compare gene expression profiles of DRG neurons either with or lacking NGF signaling.
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| Overall design |
For the first screen comparing DRGs of Bax−/− and Ngf−/−; Bax−/− mice, DRGs were dissected and pooled from E14.5 Ngf−/−; Bax−/− and Bax−/− embryos. Total RNA was extracted and directly subjected to microarray analysis. For the second screen comparing mouse DRG explants grown in the presence or absence of NGF, E13.5 mouse DRG explants were cultured for two days with 50 ng/ml of either NGF or NT3. Total RNA was extracted and then subjected to microarray analysis.
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| Contributor(s) |
Mandai K, Ginty DD |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 24, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
David D Ginty |
| E-mail(s) |
dginty@jhmi.edu
|
| Phone |
410-614-9494
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| Organization name |
HHMI/Johns Hopkins Univ
|
| Department |
Neuroscience
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| Lab |
David Ginty
|
| Street address |
725 N. Wolf ST
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (4)
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| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
| GPL82 |
[MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array |
| GPL83 |
[MG_U74Cv2] Affymetrix Murine Genome U74 Version 2 Array |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (16)
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| GSM629408 |
E14.5 DRG from Bax knockout mice A |
| GSM629409 |
E14.5 DRG from Bax knockout mice B |
| GSM629410 |
E14.5 DRG from NGF; Bax double knockout mice A |
| GSM629411 |
E14.5 DRG from NGF; Bax double knockout mice B |
| GSM629412 |
E13.5 mouse DRG cultured for 48 hours with NGF 1 chip 1 |
| GSM629413 |
E13.5 mouse DRG cultured for 48 hours with NGF 1 chip 2 |
| GSM629414 |
E13.5 mouse DRG cultured for 48 hours with NGF 1 chip 3 |
| GSM629415 |
E13.5 mouse DRG cultured for 48 hours with NGF 2 chip 1 |
| GSM629416 |
E13.5 mouse DRG cultured for 48 hours with NGF 2 chip 2 |
| GSM629417 |
E13.5 mouse DRG cultured for 48 hours with NGF 2 chip 3 |
| GSM629418 |
E13.5 mouse DRG cultured for 48 hours with NT3 1 chip 1 |
| GSM629419 |
E13.5 mouse DRG cultured for 48 hours with NT3 1 chip 2 |
| GSM629420 |
E13.5 mouse DRG cultured for 48 hours with NT3 1 chip 3 |
| GSM629421 |
E13.5 mouse DRG cultured for 48 hours with NT3 2 chip 1 |
| GSM629422 |
E13.5 mouse DRG cultured for 48 hours with NT3 2 chip 2 |
| GSM629423 |
E13.5 mouse DRG cultured for 48 hours with NT3 2 chip 3 |
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| Relations |
| BioProject |
PRJNA134263 |
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