|
| Status |
Public on Nov 12, 2011 |
| Title |
Initiation Pausing of 80S Ribosome on mRNA Is Governed by 5'UTR and Responsive to mTORC1 Signaling (RNA-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Recent studies have revealed that the mRNA translation is punctuated by ribosomal pauses through the body of transcripts. However, little is known about its physiological significance and regulatory aspects. Here we present a multi-dimensional ribosome profiling approach to quantify the dynamics of initiation and elongation of 80S ribosomes across the entire transcriptome in mammalian cells. We show that a subset of transcripts have a significant pausing of 80S ribosome around the start codon, creating a major barrier to the commitment of translation elongation. Intriguingly, genes encoding ribosome proteins themselves exhibit an exceptionally high initiation pausing on their transcripts. Our studies also reveal that the initiation pausing is dependent on the 5’ untranslated region (5’ UTR) of mRNAs and subject to the regulation of mammalian target of rapamycin complex 1 (mTORC1). Thus, the initiation pausing of 80S ribosome represents a novel regulatory step in translational control mediated by nutrient signaling pathway.
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| Overall design |
Monitor the translational status of transcriptome in mammalian cells under different conditions
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| |
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| Contributor(s) |
Han Y, Liu B, Jiang H, Sun J, Gu Z, Qian S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 27, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Shu-Bing Qian |
| E-mail(s) |
sq38@cornell.edu
|
| Phone |
(607)254-3397
|
| URL |
http://qian.human.cornell.edu
|
| Organization name |
Cornell University
|
| Department |
Division of Nutritional Sciences
|
| Street address |
301 Biotech Bldg.
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
|
| Samples (21)
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| This SubSeries is part of SuperSeries: |
| GSE25627 |
Initiation Pausing of 80S Ribosome on mRNA Is Governed by 5’UTR and Responsive to mTORC1 Signaling |
|
| Relations |
| SRA |
SRP004708 |
| BioProject |
PRJNA142427 |
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