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| Status |
Public on Nov 30, 2010 |
| Title |
Genomic Profiling of HMGN1 Reveals an Association with Chromatin at Regulatory Regions |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
High mobility group (HMG) proteins interact with other architectural proteins to regulate chromatin structure. We performed genome-wide mapping experiments to examine the distribution of HMGN1 across the human genome. We discovered that HMGN1 proteins are enriched at regulatory regions such as DNase I HS sites, distal transcription factor binding sites and promoters of actively transcribed genes.
YY1, the Yin Yang 1 transcription factor, is a ubiquitous transcription factor that functions either as a transcriptional activator or a repressor, depending on its interacting partner. To test the preferential localization of HMGN1 with active regulatory regions, we examined the association of transcription factor YY1 and HMGN1 in CD4+ T cells.
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| Overall design |
Examination of the genomic profile of HMGN1 and the relationship with regulatory elements in CD4+ T cells.
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| Contributor(s) |
Cuddapah S, Schones D, Cui K, Roh T, Barski A, Wei G, Rochman M, Bustin M, Zhao K |
| Citation(s) |
21173166 |
| Submission date |
Nov 29, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Dustin E Schones |
| E-mail(s) |
dschones@coh.org
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| Organization name |
City of Hope
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| Street address |
1500 E Duarte Rd.
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| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
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| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
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| Samples (3) |
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| Relations |
| SRA |
SRP004717 |
| BioProject |
PRJNA133869 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE25674_RAW.tar |
371.6 Mb |
(http)(custom) |
TAR (of BED) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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