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| Status |
Public on Dec 01, 2010 |
| Title |
Comparative analysis of mouse cardiac gene expression: diet, sex, and disease |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The perception that soy food products and dietary supplements will have beneficial effects on heart health has led to a massive consumer market. However, we have previously noted that diet has a profound effect on disease progression in a genetic model of hypertrophic cardiomyopathy (HCM). In this model, a soy-based diet negatively impacts cardiac function in male mice.
Given the frequent correlation between functional changes and transcriptional changes, we investigated the effect of diet (soy- vs. milk-based) on cardiac gene expression and how it relates to the additional factors of sex and disease. We found that gene expression in the heart is altered more by diet than by sex or an inherited disease. We also found that the healthy male heart may be sensitized to dietary perturbations of gene expression in that it displays a gene expression profile more similar to diseased hearts than to healthy female hearts. These observations may in part account for divergence in HCM phenotypes between males and females and between diets.
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| Overall design |
Hearts from male and female wild-type or HCM C57BL/6 mice fed either a soy or casein-based diet were excised at 2 months of age. Total RNA was extracted from left ventricles. Biotin-labeled amplified RNA was hybridized to MG_U74Av2 Affymetrix microarrays.
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| Contributor(s) |
Luczak ED, Barthel KK, Stauffer BL, Konhilas JP, Cheung TH, Leinwand LA |
| Citation(s) |
21487031 |
| Submission date |
Nov 30, 2010 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Leslie Leinwand |
| E-mail(s) |
Leslie.Leinwand@colorado.edu
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| Organization name |
University of Colorado at Boulder
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| Department |
MCD Biology and BioFrontiers Institute
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| Street address |
3415 Colorado Ave
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| City |
Boulder |
| State/province |
CO |
| ZIP/Postal code |
80303 |
| Country |
USA |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (30)
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| GSM631253 |
heart, male soy wt, rep4 |
| GSM631254 |
heart, male casein wt, rep1 |
| GSM631255 |
heart, male casein wt, rep2 |
| GSM631256 |
heart, male casein wt, rep3 |
| GSM631257 |
heart, male casein wt, rep4 |
| GSM631258 |
heart, male soy HCM, rep1 |
| GSM631259 |
heart, male soy HCM, rep2 |
| GSM631260 |
heart, male soy HCM, rep3 |
| GSM631261 |
heart, male casein HCM, rep1 |
| GSM631262 |
heart, male casein HCM, rep2 |
| GSM631263 |
heart, male casein HCM, rep3 |
| GSM631264 |
heart, male casein HCM, rep4 |
| GSM631265 |
heart, female soy wt, rep1 |
| GSM631266 |
heart, female soy wt, rep2 |
| GSM631267 |
heart, female soy wt, rep3 |
| GSM631268 |
heart, female soy wt, rep4 |
| GSM631269 |
heart, female casein wt, rep1 |
| GSM631270 |
heart, female casein wt, rep2 |
| GSM631271 |
heart, female casein wt, rep3 |
| GSM631272 |
heart, female casein wt, rep4 |
| GSM631273 |
heart, female soy HCM, rep1 |
| GSM631274 |
heart, female soy HCM, rep2 |
| GSM631275 |
heart, female soy HCM, rep3 |
| GSM631276 |
heart, female casein HCM, rep1 |
| GSM631277 |
heart, female casein HCM, rep2 |
| GSM631278 |
heart, female casein HCM, rep3 |
| GSM631279 |
heart, female casein HCM, rep4 |
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| Relations |
| BioProject |
PRJNA134111 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE25700_RAW.tar |
82.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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