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Series GSE25707 Query DataSets for GSE25707
Status Public on Jan 03, 2011
Title Synergistic activation by p38MAPK and glucocorticoid signaling mediates induction of M2-like tumor-associated macrophages expressing the novel CD20 homolog MS4A8A
Organism Mus musculus
Experiment type Expression profiling by array
Summary Analysis of bone marrow derived macrophages (BMDM) incubated with dexamethasone&IL4 (Dexa+IL4), B16F1 tumor conditioned medium (cmB16), and B16F1 tumor conditioned medium supplemented with dexamethasone&IL4 (cmB16+dexa+IL4). Results allow detection of genes that require synergistic stimulation of tumor factors and Th2 cytokines.
 
Overall design Bone marrow cells were isolated from the tibias and femurs of C57BL/6 mice. M-CSF at a concentration of 30 ng/ml was added to the culture medium [DMEM, 10% FCS, and penicillin/streptomycin]. After 4 days, the culture medium was replaced by fresh DMEM or B16F1 conditioned medium. As indicated, IL-4 (10 ng/ml), dexamethasone (5 × 10−7M), Iwas added. Cells were harvested 3 days later for RNA isolation.
 
Contributor(s) Schmieder A, Schledzewski K, Michel J, Tuckermann JP, Tome L, Sticht C, Gkaniatsou C, Nicolay JP, Demory A, Faulhaber J, Kzhyshkowska J, Géraud C, Goerdt S
Citation(s) 20824698
Submission date Nov 30, 2010
Last update date Jun 21, 2012
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platforms (1)
GPL6526 [Mouse430_2_Mm_UG] Affymetrix GeneChip Mouse Genome 430 2.0 Array [Brainarray Version 9]
Samples (9)
GSM631421 BMDM stimulated with dexa+IL4, rep1
GSM631422 BMDM stimulated with cmB16F1 and dexa+IL4, rep1
GSM631423 BMDM stimulated with cmB16F1, rep1
Relations
BioProject PRJNA133823

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Supplementary file Size Download File type/resource
GSE25707_RAW.tar 54.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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