|
| Status |
Public on Feb 05, 2011 |
| Title |
[E-MTAB-345] ChIP-Seq of human MCF-7 cells with anti-ERalpha following estrogen treatment |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We performed chromatin immunoprecipitation (ChIP) with overnight with antibodies against the C-terminus (HC-20, from Santa Cruz Biotechnology, Europe) or the N-terminus (anti-Estrogen Receptor 18-32, from SigmaAldrich, Italy) of human ER-alpha with or without estrogen treatment for 45 minutes on TAP-ER-beta cells, and the immunoprecipitated DNA was sequenced with the Illumina/Solexa Genome Analyzer. (Note: Raw data files not provided by submitter)
Publication Title: Global analysis of ERbeta binding to human breast cancer cell genome
Publication Author List: Grober OMV, Mutarelli M, Paris O, Giurato G, De Filippo MR, Cicatiello L, Ravo M, Ferraro L, Nassa G, Tarallo R, Papa MF, Benes V, Alessandro Weisz.
ArrayExpress Release Date: 2010-10-05
Person Roles: submitter
Person Last Name: Weisz
Person First Name: Alessandro
Person Mid Initials:
Person Email: alessandro.weisz@unina2.it
Person Phone:
Person Address:
Person Affiliation: Second University of Naples
|
| |
|
| Overall design |
Experimental Design: compound_treatment_design
Experimental Design: in_vivo_design
Experimental Design: binding_site_identification_design
Experimental Design: high_throughput_sequencing_design
Experimental Factor Name: COMPOUND
Experimental Factor Name: IMMUNOPRECIPITATE
Experimental Factor Name: GENOTYPE
Experimental Factor Name: DOSE
Experimental Factor Type: compound
Experimental Factor Type: immunoprecipitate
Experimental Factor Type: genotype
Experimental Factor Type: dose
Quality Control Type: real_time_PCR_quality_control
Quality Control Type: technical_replicate
|
| |
|
| Citation(s) |
21235772 |
| Submission date |
Dec 01, 2010 |
| Last update date |
Dec 03, 2018 |
| Organization |
European Bioinformatics Institute |
| E-mail(s) |
miamexpress@ebi.ac.uk
|
| Lab |
ArrayExpress
|
| Street address |
Wellcome Trust Genome Campus
|
| City |
Hinxton |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB10 1SD |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
|
| Samples (8)
|
| GSM632885 |
[E-MTAB-345] TAP-ER-beta_+E2_ER-alpha IP sequence Performer: Illumina_Hayward |
| GSM632886 |
[E-MTAB-345] TAP-ER-beta_+E2_ER-alpha IP sequence Performer: EMBL_Heidelberg |
| GSM632887 |
[E-MTAB-345] TAP-ER-beta_-E2_ER-alpha IP sequence Performer: Illumina_Hayward |
| GSM632888 |
[E-MTAB-345] TAP-ER-beta_-E2_ER-alpha IP sequence Performer: EMBL_Heidelberg |
| GSM632889 |
[E-MTAB-345] TAP-ER-beta_+E2_ER-beta IP sequence Performer: Illumina_Hayward |
| GSM632890 |
[E-MTAB-345] TAP-ER-beta_+E2_ER-beta IP sequence Performer: EMBL_Heidelberg |
| GSM632891 |
[E-MTAB-345] TAP-ER-beta_-E2_ER-beta IP sequence Performer: Illumina_Hayward |
| GSM632892 |
[E-MTAB-345] TAP-ER-beta_-E2_ER-beta IP sequence Performer: EMBL_Heidelberg |
|
| Relations |
| ArrayExpress |
E-MTAB-345 |
| BioProject |
PRJNA134073 |
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