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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 28, 2005 |
| Title |
promiscuous gene expression in the mouse thymus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression in different thymic stromal cells and subsets thereof was analyzed in 6-12 week old wild type (C57BL/6) and Aire knock-out (mixed background) mice. Thymic stromal cells were purified by sequential enzymatic digestion (collagenase, collagenase/dispase and trypsin) followed by gradient centrifugation and FACS sorting. Sort criteria were as follows: dendritic cells (CD11c+, F4/80 -), macrophages (F4/80+, CD11c-), cTECs (CD45–/lo, CDR1/Ly51+, Ep-CAM+) and mTECs (CD45–/lo, CDR1/Ly51–, Ep-CAM+). mTECs of wild-type and Aire knock-out mice were further subdivided according to CD80 expression levels.
For microarray analysis total RNA from thymic stromal cell samples of two independent experiments was pre-amplified and biotinylated by two rounds of cDNA synthesis and in vitro transcription. Fluorescence readings were evaluated by using Microarray Suite 5.0 software.
Keywords = thymus
Keywords = promiscuous gene expression
Keywords = AIRE
Keywords: ordered
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| Contributor(s) |
Derbinski J, Gaebler J, Brors B, Tierling S, Jonnakuty S, Hergenhahn M, Peltonen L, Walter J, Kyewski B |
| Citation(s) |
15983066 |
| Submission date |
Apr 27, 2005 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Benedikt Brors |
| E-mail(s) |
b.brors@dkfz-heidelberg.de
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| Phone |
+49-6221-42 3614
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| Organization name |
German Cancer Research Center (DKFZ)
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| Street address |
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| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA91995 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE2585_RAW.tar |
45.9 Mb |
(http)(custom) |
TAR (of CEL) |
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