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Series GSE25870 Query DataSets for GSE25870
Status Public on Jan 01, 2012
Title Human Beta-Defensin 3 attenuates the pro-inflammatory effects of LPS at 1 hour
Organism Mus musculus
Experiment type Expression profiling by array
Summary We examine the global effect of hBD3 on transcription in TLR4-stimulated macrophages and for the first time show that hBD3 inhibits the transcription of critical pro-inflammatory genes.
Among the repressed genes we detect significant enrichment of groups involved in the positive regulation of NFkappaB including components of Toll-like receptor signaling pathways.
 
Overall design Bone marrow derived macrophages from 3 separate mice were used for each treatment condition. ie macrophages from 3 mice were left untreated, macrophages from 3 mice were treated with LPS, macrophages from 3 mice were treated with LPS and hBD3, macrophages from 3 mice were treated with hBD3. We examined the differences in gene expression between each treatment group.
 
Contributor(s) Semple F, Dorin JR, Webb S, Grimes GR, Semple CA
Citation(s) 21809339
Submission date Dec 06, 2010
Last update date Aug 14, 2019
Contact name Graeme Richard Grimes
E-mail(s) graeme.grimes@igmm.ed.ac.uk
Organization name University of Edinburgh, The Institute of Genetics and Molecular Medicine
Department Human Genetics Unit
Lab Bioinformatics
Street address Western General Hospital, Crewe Road
City Edinburgh
ZIP/Postal code EH4 2XU
Country United Kingdom
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (12)
GSM635212 Untreated rep1
GSM635213 HBD3 rep 1
GSM635214 LPS rep 1
This SubSeries is part of SuperSeries:
GSE25873 Effect of hBD3 on transcription in TLR4-stimulated macrophages
Relations
BioProject PRJNA142471

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25870_RAW.tar 3.1 Mb (http)(custom) TAR
GSE25870_non-normalized_data.txt.gz 2.0 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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