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| Status |
Public on Aug 08, 2011 |
| Title |
Delineation of AR oncogenic functions in hepatocellular carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
The goal of this study was to delineate the important AR direct target genes that mediate the oncogenic properties of AR in HCC. The AR direct target genes in two HCC cell lines were identified by chromatin immunoprecipitation microarray (ChIP-chip) analysis and later confirmed by independent ChIP-PCR. The functions of the target genes were further examined.
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| Overall design |
Two human HCC cell lines, Huh7 and PLC/PRF/5 (PLC5), were grown in DMEM supplemented with 10% fetal bovine serum. ChIP assays were performed using anti-AR antibody. The immunoprecipitated and input DNA were used to probe the Agilent human ChIP-chip arrays.
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| |
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| Contributor(s) |
Cheng AS |
| Citation(s) |
21747169 |
| Submission date |
Dec 06, 2010 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Hai Feng |
| E-mail(s) |
fogsea@163.com
|
| Phone |
852-37636104
|
| Fax |
852-21445330
|
| Organization name |
The Chinese University of Hong Kong
|
| Street address |
The Chinese University of Hong Kong
|
| City |
Hong Kong |
| ZIP/Postal code |
852 |
| Country |
Hong Kong |
| |
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| Platforms (2) |
| GPL4124 |
Agilent-014706 Human Promoter ChIP-on-Chip Set 244K, Microarray 1 of 2 G4489A (Feature Number version) |
| GPL4125 |
Agilent-014707 Human Promoter ChIP-on-Chip Set 244K, Microarray 2 of 2 G4489A (Feature Number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA135817 |
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