 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 27, 2011 |
| Title |
Gene Expression Profiling of Human Embryonic Neural Stem Cells and Dopaminergic Neurons from Adult Human Substantia Nigra |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Our goal was to develop a list of genes that could be used effectively to highlight the genomic profiling of human embryonic neural stem cells (hENSCs), and to identify genes involved in the process of their differentiation to dopaminergic (DA) neurons. Our results showed that Agilent's Whole Human Genome Oligonucleotide Microarray permits the monitoring of at least 41000 genes as cells differentiate. In this study, we identified 13525 genes to be differentially expressed between undifferentiated hENSC and DA cells isolated from human substansia nigra. Approximately 3737 genes were up-regulated in the embryonic NSC, and 4116 genes were up-regulated in DA cells. Careful analysis of the emerged data using the web-accessible program named Database for Annotation, Visualization and Integrated Discovery (DAVID) has permitted us to distinguish several genes and pathways that are involved in dopaminergic differentiation, and to identify the crucial signaling pathways that direct the process of differentiation. Our study elucidated that genes related to midbrain development, such as Nr4a2 (nuclear receptor subfamily 4, group A, member 2, Nurr1) and En1 (engrailed 1), were increased to 3.76- and 6.41-folds, respectively. In addition, the transcriptions of the genes for DA neuron phenotype, such as Ddc (doapmine decarboxylase, AADC), Slc6a3 (solute carrier family 6, member 3, DAT), and Th (tyrosine hydroxylase), were significantly increased to 8.08, 4.01, and 5.91, respectively. As data accumulate with different populations and different methods of differentiation, one will perhaps be able to identify the key regulators and biomarkers that may allow selective selection of limited number of genes or transcription factors to be used for direct reprogramming of NSC into DA cells, with an ultimate goal of obtaining different types of allogenic neurons including personalized DA neurons to be used in replacement therapy for neurodegenerative diseases such as Parkinson's disease (PD).
|
| |
|
| Overall design |
Two-condition experiment: hENSC S vs. DA cells. Biological replicates: 2 hENSC, and 2 DA neuron samples from human substantia nigra cells.
|
| |
|
| Contributor(s) |
Marei H, Deng W, Afifi N |
| Citation(s) |
22163301 |
| Submission date |
Dec 08, 2010 |
| Last update date |
May 14, 2019 |
| Contact name |
Hany El Sayed Marei |
| E-mail(s) |
hany800@yahoo.com
|
| Phone |
0020121650355
|
| Fax |
0020502379952
|
| URL |
http://www.vetmansoura.com
|
| Organization name |
Fac. Vet Med
|
| Department |
Histology
|
| Street address |
Gomhoraya
|
| City |
Mansoura |
| State/province |
Dakahlaya |
| ZIP/Postal code |
35116 |
| Country |
Egypt |
| |
|
| Platforms (1) |
| GPL13497 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version) |
|
| Samples (4)
|
| GSM636910 |
Human Embyronic Neural Stem Cell, Replicate 1 |
| GSM636911 |
Human Embyronic Neural Stem Cell, Replicate 2 |
| GSM636912 |
Dopaminergic Neurons from Human Substantia nigra, Replicate 1 |
| GSM636913 |
Dopaminergic Neurons from Human Substantia nigra, Replicate 2 |
|
| Relations |
| BioProject |
PRJNA135681 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE25931_RAW.tar |
8.6 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...