|
| Status |
Public on Jan 13, 2011 |
| Title |
In vitro and in vivo analysis of hypoxic gene expression in rat gliomas |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
In vivo profiling of hypoxic gene expression in gliomas using the hypoxia marker EF5 and laser-capture microdissection
We have employed the hypoxia marker EF5 coupled with laser capture microdissection to isolate RNA from viable hypoxic and normoxic regions of 9L experimental gliomas.
|
| |
|
| Overall design |
We report the identification of the hypoxic gene expression profile in a solid tumor using a prototypical hypoxia marker EF5, Laser Capture Microdissection (LCM) and Microarray Gene Expression Profiling (EF5-LCM-MGEP). We have utilized LCM to isolate total RNA from normoxic and hypoxic regions in the rat 9L glioma tumor model grown in its isogenic host (Fischer rat). Microarray analysis of the isolated RNA generated an in vivo hypoxia expression profile. For comparison, we also analyzed RNA samples isolated from rat 9L glioma cells in culture exposed to hypoxia or normoxia.
|
| |
|
| Contributor(s) |
Koumenis C, Maity A, Koch C |
| Citation(s) |
21266355 |
| Submission date |
Dec 09, 2010 |
| Last update date |
Nov 06, 2013 |
| Contact name |
Constantinos Koumenis |
| E-mail(s) |
koumenis@xrt.upenn.edu
|
| Organization name |
University of Pennsylvania
|
| Department |
Radiation Oncology
|
| Street address |
3620 Hamilton Walk
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19146 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA135523 |
Less...
More...