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| Status |
Public on Aug 17, 2011 |
| Title |
Expression data from C2C12 murine myoblast cells infected with an inducible Msx1-ER fusion protein |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The spatial and temporal control of gene expression during development requires the concerted actions of sequence-specific transcriptional regulators and epigenetic chromatin modifiers, which are thought to function within precise nuclear compartments. However, how these activities are coordinated within the dynamic context of the nuclear environment is still largely unresolved. Here we show that transcriptional repression by the Msx1 homeoprotein coordinates recruitment of Polycomb to genomic targets with localization to the nuclear periphery. Using genome-wide ChIP-Seq analyses to identify genomic binding sites for Msx1, we find that repressed target genes are enriched at the nuclear periphery in myoblast cells. We further show that the interaction of Msx1 with the Polycomb repressive complex PRC2 is required for transcriptional repression and regulation of myoblast differentiation, and promotes increased tri-methylation of lysine 27 on histone H3 (H3K27me3) at Msx1 target genes. Furthermore, Msx1 genomic binding promotes the dynamic spatial redistribution of the H3K27me3 repressive mark to the nuclear periphery in developing embryos in vivo. Thus, our findings suggest a hitherto unappreciated spatial coordination of transcription factor binding, Polycomb recruitment, and subnuclear localization in regulation of developmentgene expression programs.
In order to identify genes regulated by Msx1, we infected C2C12 myoblast cells with a retrovirus expressing a tamoxifen-regulated Msx1 protein, Msx1-ER (or with empty vector as a control), followed by induction with 0.2 nM of tamoxifen or vehicle (DMSO) for 6 hours. Regulated genes were identified as those that changed in expression upon tamoxifen induction of the Msx1-ER protein but did not change in the empty vector control.
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| Overall design |
Experiments were performed in triplicate for each of the four experimental conditions (Msx1-ER + tamoxifen, Msx1-ER - tamoxifen, empty vector control + tamoxifen, empty vector control - tamoxifen), for a total of 12 independent array samples.
This submission represents the transcriptome component of the study.
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| Contributor(s) |
Wang J, Kumar RM, Biggs VJ, Lee H, Chen Y, Kagey MH, Young RA, Abate-Shen C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 11, 2010 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Richard A Young |
| E-mail(s) |
young_computation@wi.mit.edu
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| Phone |
617-258-5219
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| Organization name |
Whitehead Institute for Biomedical Research
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| Lab |
Young Lab
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| Street address |
9 Cambridge Center
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (12)
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| GSM638408 |
Empty vector, no tamoxifen, biological rep1 |
| GSM638409 |
Empty vector, no tamoxifen, biological rep2 |
| GSM638410 |
Empty vector, no tamoxifen, biological rep3 |
| GSM638411 |
Empty vector, plus tamoxifen, biological rep1 |
| GSM638412 |
Empty vector, plus tamoxifen, biological rep2 |
| GSM638413 |
Empty vector, plus tamoxifen, biological rep3 |
| GSM638414 |
Msx1-ER vector, no tamoxifen, biological rep1 |
| GSM638415 |
Msx1-ER vector, no tamoxifen, biological rep2 |
| GSM638416 |
Msx1-ER vector, no tamoxifen, biological rep3 |
| GSM638417 |
Msx1-ER vector, plus tamoxifen, biological rep1 |
| GSM638418 |
Msx1-ER vector, plus tamoxifen, biological rep2 |
| GSM638419 |
Msx1-ER vector, plus tamoxifen, biological rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE27089 |
The Msx1 homeoprotein recruits Polycomb to the nuclear periphery during development |
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| Relations |
| BioProject |
PRJNA142525 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26021_RAW.tar |
29.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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