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Series GSE26033 Query DataSets for GSE26033
Status Public on Feb 25, 2011
Title A Genome-wide Study of DNA Methylation Patterns and Gene Expression Levels in Multiple Human and Chimpanzee Tissues
Platform organism Homo sapiens
Sample organisms Pan troglodytes; Homo sapiens
Experiment type Methylation profiling by array
Summary It has long been hypothesized that changes in gene regulation have played an important role in primate evolution. However, despite the wealth of comparative gene expression data, there are still only few studies that focus on the mechanisms underlying inter-primate differences in gene regulation. In particular, we know relatively little about the degree to which changes in epigenetic profiles might explain differences in gene expression levels between primates. To this end, we studied DNA methylation and gene expression levels in livers, hearts, and kidneys from multiple humans and chimpanzees.
 
Overall design Genome wide DNA methylation profiling of heart, kidney, and liver samples from humans and chimpanzees. The Illumina Infinium 27K Human DNA Methylation BeadChip v1.2 was used to compare DNA methylation profiles across approximately 10,575 CpG sites in human and chimpanzee tissue samples. Samples included 6 human livers, 6 human kidneys, 6 human heart, 6 chimpanzee livers, 6 chimpanzee kidneys, and 6 chimpanzee hearts. Two techical replicates of bisulfite converted DNA from each of the 36 samples was hybridized to the Illumina HumanMethylation27 Beadchip. We limited our analysis to the 10,575 probes that were a perfect sequence match to both the human and chimpanzee genomes and which were associated with genes for which we had previously collected expression measurements across the three tissues.
 
Contributor(s) Pai AA, Bell JT, Marioni JC, Pritchard JK, Gilad Y
Citation(s) 21383968
Submission date Dec 13, 2010
Last update date Jan 02, 2015
Contact name Athma A Pai
E-mail(s) athma@uchicago.edu
Organization name University of Chicago
Street address 920 E. 58th Street
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
 
Platforms (1)
GPL8490 Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2)
Samples (71)
GSM639308 genomic DNA from human liver 1 (replicate A)
GSM639309 genomic DNA from human liver 1 (replicate B)
GSM639310 genomic DNA from human liver 2 (replicate A)
Relations
BioProject PRJNA135501

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26033_RAW.tar 5.8 Mb (http)(custom) TAR
GSE26033_non-normalized.txt.gz 10.6 Mb (ftp)(http) TXT
Processed data included within Sample table
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