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Status |
Public on Dec 15, 2010 |
Title |
Epidermal differentiation: basal vs. suprabasal keratinocytes |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
In epidermal differentiation basal keratinocytes detach from the basement membrane, stop proliferating, and express a new set of structural proteins and enzymes, which results in an impermeable protein/lipid barrier that protects us. To define the transcriptional changes essential for this process, we purified large quantities of basal and suprabasal cells from human epidermis, using the expression of b4 integrin as the discriminating factor. The expected expression differences in cytoskeletal, cell cycle and adhesion genes confirmed the effective separation of the cell populations. Using DNA microarray chips, we comprehensively identify the differences in genes expressed in basal and differentiating layers of the epidermis, including the ECM components produced by the basal cells, the proteases in both the basal and suprabasal cells, and the lipid and steroid metabolism enzymes in suprabasal cells responsible for the permeability barrier. We identified the signaling pathways specific for the two populations, and found two previously unknown paracrine and one juxtacrine signaling pathway operating between the basal and suprabasal cells. Furthermore, using specific expression signatures, we identified a new set of late differentiation markers and mapped their chromosomal loci, as well as a new set of melanocyte-specific markers. The data represent a quantum jump in understanding the mechanisms of epidermal differentiation. Basal keratinocytes are defined as integrin b4-positive. Suprabasal sample contains all melanocytes as well.
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Overall design |
Basal (integrin b4+), suprabasal (b4-) and total epidermal keratinocytes. In duplicate, from 2 independent donors, breast reduction surgery.
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Contributor(s) |
Blumenberg M, Gazel A, Radoja N, Banno T, Yano S |
Citation(s) |
16822832 |
Submission date |
Dec 14, 2010 |
Last update date |
Dec 06, 2018 |
Contact name |
Miroslav Blumenberg |
E-mail(s) |
miroslav.blumenberg@nyumc.org
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Phone |
212 263-5924
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Organization name |
NYU School of Medicine
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Department |
Dermatology
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Lab |
Blumenberg
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Street address |
455 First Ave #874
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City |
New York |
State/province |
NY |
ZIP/Postal code |
10016 |
Country |
USA |
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Platforms (1) |
GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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Samples (7)
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GSM639879 |
Total epidermal cells, all layers Sample 1 |
GSM639880 |
Basal epidermal cells, Sample 1 |
GSM639881 |
Suprabasal epidermal cells, Sample 1 |
GSM639882 |
Total epidermal cells, all layers Sample 2 |
GSM639883 |
Basal epidermal cells, Sample 2 |
GSM639884 |
Suprabasal epidermal cells, Sample 2 |
GSM639885 |
Suprabasal epidermal cells, Sample 2 replicate |
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Relations |
BioProject |
PRJNA135569 |
Supplementary file |
Size |
Download |
File type/resource |
GSE26059_RAW.tar |
16.6 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
Processed data included within Sample table |
Processed data provided as supplementary file |
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