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| Status |
Public on May 27, 2011 |
| Title |
Gene Expression Analysis of Fezf1 Mutant Main Olfactory Epithelium |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Mice lacking the transcription factor Fezf1 exhibit defects in the structural and molecular organiztion of their olfactory system. To invetigate this at the level of gene expression, we isolated Fezf1 expressing cells by FACS from the MOE of Fezf1+/- or Fezf1-/- animals and compared their gene expression profiles.
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| Overall design |
The targeted Fezf1 allele contains an EGFP coding sequence under control of the endogenous Fezf1 promoter. We utilized this to isolate Fezf1+/- or Fezf1 -/- cells from the microdissected MOE tissue of E18.5 mouse embryos. Total RNA was isolated from these cells and gene expression examined by Affymetrix 1.0 ST array.
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| Contributor(s) |
Eckler MJ, Chen B |
| Citation(s) |
21452247 |
| Submission date |
Dec 14, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Matthew Joseph Eckler |
| E-mail(s) |
eckler@biology.ucsc.edu
|
| Organization name |
UCSC
|
| Department |
MCDB
|
| Lab |
Bin Chen
|
| Street address |
434 Sinsheimer Labs, UCSC
|
| City |
Santa Cruz |
| State/province |
CA |
| ZIP/Postal code |
95064 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA135573 |
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