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| Status |
Public on Dec 18, 2013 |
| Title |
Gene analysis of flagellin-treated nasal mucosa of Duox2-/- mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To evaluate the roles of DUOX2 in flagellin- induced inflammatory response in mouse nasal mucosa. Wild type (Duox2+/+) and Duox2 knockout (Duox2-/-) mice were stimulated with 1 μg/ml of flagellin for 4h.
422 genes (> 2 fold) were up-regulated in nasal mucosa of Duox2+/+ that was treated with flagellin and the full list of genes is presented in Supplemental Table II. These genes included the following defense- and immune response-related genes : Cytokine/chemokine-related genes (CCL20, CCR2, CCR5, CXCL2, CXCL5, CXCL9, CXCL16, IL18RAP, TNFAIP2, IL1B EAR2, FPR1), Granulocyte-related genes (IL8RB, MPO, PRG2, PPBP, PRG3), interferon-related genes (IFITM6, IFI47), macrophage related genes (IL1B, S100A9), and T-cell mediated immune response related genes (H2-Q6, IL1F9). In addition, signal transduction (PPBP, OLFR60, P2RY10) and cell adhesion (SELL, SELP, ICAM1, DSG1A, DSG3, VCAM1) related genes were also increased by flagellin treatment. These genes were selected based on the biological processes and molecular functions of their gene ontology. However, the increase of inflammation and immune response related genes by flagellin treatment were diminished in the nasal mucosa of the Duox2-/- mice compared with that of Duox2+/+ mice.
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| Overall design |
Wild type (Duox2+/+) and Duox2 knockout (Duox2-/-) mice received either PBS (control) or flagellin (5 ug/ml) intranasally for 4h.
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| Contributor(s) |
Joo J, Bae YS, Yoon J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 17, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
JUNGHEE JOO |
| E-mail(s) |
bioplan@yuhs.ac
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| Phone |
82-2-2228-2667
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| Fax |
82-2-2228-7998
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| Organization name |
Yonsei University
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| Department |
College of Medicine
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| Lab |
Research Center for Natural Human Defense System,
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| Street address |
134 Shinchon-dong, Seodaemun-gu
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| City |
Seoul |
| ZIP/Postal code |
120-752 |
| Country |
South Korea |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| GSM641801 |
PBS treated nasal mucosa of wild type mouse (C56BL/6) |
| GSM641802 |
Flagellin treated nasal mucosa of wild type mouse (C56BL/6) |
| GSM641803 |
PBS treated nasal mucosa of Duox2 knockout (Duox2-/-) mouse |
| GSM641804 |
Flagellin treated nasal mucosa of Duox2 knockout (Duox2-/-) mouse |
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| Relations |
| BioProject |
PRJNA135383 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26135_RAW.tar |
16.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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