|
| Status |
Public on Feb 16, 2011 |
| Title |
Examination of inflammatory transcripts during a transfer model of type I diabetes. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
In an accompanying paper we found specific localization of diabetogenic T cells only to islets of Langerhans bearing the specific antigen. Instrumental in the specific localization was the presence of intra-islet dendritic cells bearing the β-cell-peptide-MHC complex. Here we report that the entry of diabetogenic CD4 T cells very rapidly triggered inflammatory gene expression changes in islets and vessels by up-regulating chemokines and adhesion molecules. VCAM-1 expression was notable in blood vessels and so was ICAM-1. ICAM-1 was also found on β-cells. These expression changes induced the entry of non-specific T cells that otherwise did not localize to the islets. In contrast to the entry of diabetogenic CD4 T cells, the entrance of non-specific T cells required a chemokine response and VCAM-1 expression by the islets. Interferon-gamma was important for the early gene expression changes in the islets. By microarray analysis we detected up-regulation of a group of interferon-inducible genes as early as 8 hours post T cell transfer. These studies provide a baseline to examine the development of therapeutics that can modulate islet localization of diabetogenic T cells to control this autoimmune disease.
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| |
|
| Overall design |
20 total samples
|
| |
|
| Contributor(s) |
Unanue ER, Carrero JA, Calderon B |
| Citation(s) |
21220309 |
| Submission date |
Dec 17, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Emil Unanue |
| E-mail(s) |
unanue@pathology.wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Department |
Pathology and Immunology
|
| Street address |
660 S Euclid Ave Box 8118
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (20)
|
|
| Relations |
| BioProject |
PRJNA135303 |
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