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| Status |
Public on Aug 16, 2011 |
| Title |
Healthy volunteers gene expression study of CD4 and NK lymphocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Sixteen volunteers, that were age, gender and BMI matched, were included in the study. Blood samples were taken one per each volunteer on three different days to also assess variability of gene expression markers in one individual. In all datasets variability between volunteers was higher than variability of measurables in samples collected from the same volunteer on different days. We have identified genes in individuals that are highly stable during one month period and are thus suitable as markers of individual disease/therapy progress. A list of genes with low overall variability is also available. Interestingly, data show that important components of immune response are down-regulated with BMI in T helper and NK cells. This paper also gives the first insight into the gene expression profile of T helper and NK cells in healthy population.
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| Overall design |
CD4 and NK lymphocytes were isolated with positive selection using magnetic nanoparticle based kits from StemCell Inc. (Canada) and frozen to -80°C until total RNA extraction.
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| Contributor(s) |
Gruden K, Herman A, Hren M, Rožman P, Kneževič M, Turnšek N, Hren M, Ravnikar M, Jeras M |
| Citation(s) |
22253695 |
| Submission date |
Dec 17, 2010 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Kristina Gruden |
| E-mail(s) |
kristina.gruden@nib.si
|
| Organization name |
National Institute of Biology
|
| Department |
Department of Biotechnology and Systems Biology
|
| Street address |
Vecna pot 111
|
| City |
Ljubljana |
| ZIP/Postal code |
1000 |
| Country |
Slovenia |
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| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
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| Samples (68)
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| Relations |
| BioProject |
PRJNA135283 |
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