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| Status |
Public on Mar 01, 2011 |
| Title |
The effects of EBV transformation on methylation levels |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
|
| Summary |
Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines (LCLs) provide a conveniently accessible and renewable resource for functional studies in humans. The ability to accumulate multidimensional data pertaining to the same individual cell lines, from complete genomic sequences to detailed gene regulatory profiles, further enhances the utility of LCLs as a model system. A lingering concern, however, is that the changes associated with EBV transformation of LCLs reduce the usefulness of LCLs as a surrogate model for primary tissues. To evaluate the validity of this concern, we compared global promoter CpG profiles between CD20+ primary B cells and CD3+ primary T cells sampled from six individuals. Six independent replicates of transformed LCLs were derived from each sample.
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| Overall design |
The study included 96 DNA samples: 8 different types (B cell + T cell + 6 independent LCLs per individual) x 6 individuals x 2 technical replicates (each DNA sample hybridized in duplicate).
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| Contributor(s) |
Caliskan M, Cusanovich D, Ober C, Gilad Y |
| Citation(s) |
21289059 |
| Submission date |
Dec 20, 2010 |
| Last update date |
Jan 02, 2015 |
| Contact name |
Minal Caliskan |
| Organization name |
University of Pennsylvania
|
| Department |
Genetics
|
| Street address |
415 Curie Blvd., Room 540
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL8490 |
Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2) |
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| Samples (96)
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| This SubSeries is part of SuperSeries: |
| GSE26212 |
The effects of EBV transformation on gene expression and methylation levels |
|
| Relations |
| BioProject |
PRJNA142327 |
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