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| Status |
Public on Apr 01, 2012 |
| Title |
Expression data from cytoplasmic hybrid (cybrid) and rho0 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Mitochondria have been implicated in insulin resistance and beta cell dysfunction, both of which comprise the core pathophysiology of type 2 diabetes mellitus (T2DM). It has also recently been found that mtDNA haplogroups are distinctively associated with susceptibility to T2DM at least in Koreans and Japanese.
To investigate the functional consequences of different mtDNA, we compared gene expression profiles between cybrid clones harboring three different mtDNA haplogroups (D5, F, and N9a). To produce hybrid clones, we fused mtDNA-depleted osteosarcoma cell line (143B TK- rho0) with nucleus-lacking platelets from twelve donors harboring the three haplogroups.
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| Overall design |
A total of twelve cybrid clones from the three mtDNA haplogroups were obtained: D5 (n=3), F (n=5), and N9a (n=4). For each clone, four technical replicates were obtained and hybridized to the array. For rho0 cell, six technical replicates were obtained and hybridized to the array.
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| Contributor(s) |
Cho YM, Hwang S |
| Citation(s) |
21765942 |
| Submission date |
Dec 21, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Seungwoo Hwang |
| E-mail(s) |
swhwang10@yahoo.com
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| Phone |
82-42-879-8544
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| Organization name |
Korea Research Institute of Bioscience and Biotechnology
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| Department |
Korean Bioinformation Center
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| Street address |
52 Eoeun-dong Yuseong-gu
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| City |
Daejeon |
| ZIP/Postal code |
305-806 |
| Country |
South Korea |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (54)
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| GSM644180 |
Cybrid from haplogroup D5, biological rep 1, technical rep 1 |
| GSM644181 |
Cybrid from haplogroup D5, biological rep 1, technical rep 2 |
| GSM644182 |
Cybrid from haplogroup D5, biological rep 1, technical rep 3 |
| GSM644183 |
Cybrid from haplogroup D5, biological rep 1, technical rep 4 |
| GSM644184 |
Cybrid from haplogroup D5, biological rep 2, technical rep 1 |
| GSM644185 |
Cybrid from haplogroup D5, biological rep 2, technical rep 2 |
| GSM644186 |
Cybrid from haplogroup D5, biological rep 2, technical rep 3 |
| GSM644187 |
Cybrid from haplogroup D5, biological rep 2, technical rep 4 |
| GSM644188 |
Cybrid from haplogroup D5, biological rep 3, technical rep 1 |
| GSM644189 |
Cybrid from haplogroup D5, biological rep 3, technical rep 2 |
| GSM644190 |
Cybrid from haplogroup D5, biological rep 3, technical rep 3 |
| GSM644191 |
Cybrid from haplogroup D5, biological rep 3, technical rep 4 |
| GSM644192 |
Cybrid from haplogroup F, biological rep 1, technical rep 1 |
| GSM644193 |
Cybrid from haplogroup F, biological rep 1, technical rep 2 |
| GSM644194 |
Cybrid from haplogroup F, biological rep 1, technical rep 3 |
| GSM644195 |
Cybrid from haplogroup F, biological rep 1, technical rep 4 |
| GSM644196 |
Cybrid from haplogroup F, biological rep 2, technical rep 1 |
| GSM644197 |
Cybrid from haplogroup F, biological rep 2, technical rep 2 |
| GSM644198 |
Cybrid from haplogroup F, biological rep 2, technical rep 3 |
| GSM644199 |
Cybrid from haplogroup F, biological rep 2, technical rep 4 |
| GSM644200 |
Cybrid from haplogroup F, biological rep 3, technical rep 1 |
| GSM644201 |
Cybrid from haplogroup F, biological rep 3, technical rep 2 |
| GSM644202 |
Cybrid from haplogroup F, biological rep 3, technical rep 3 |
| GSM644203 |
Cybrid from haplogroup F, biological rep 3, technical rep 4 |
| GSM644204 |
Cybrid from haplogroup F, biological rep 4, technical rep 1 |
| GSM644205 |
Cybrid from haplogroup F, biological rep 4, technical rep 2 |
| GSM644206 |
Cybrid from haplogroup F, biological rep 4, technical rep 3 |
| GSM644207 |
Cybrid from haplogroup F, biological rep 4, technical rep 4 |
| GSM644208 |
Cybrid from haplogroup F, biological rep 5, technical rep 1 |
| GSM644209 |
Cybrid from haplogroup F, biological rep 5, technical rep 2 |
| GSM644210 |
Cybrid from haplogroup F, biological rep 5, technical rep 3 |
| GSM644211 |
Cybrid from haplogroup F, biological rep 5, technical rep 4 |
| GSM644212 |
Cybrid from haplogroup N9a, biological rep 1, technical rep 1 |
| GSM644213 |
Cybrid from haplogroup N9a, biological rep 1, technical rep 2 |
| GSM644214 |
Cybrid from haplogroup N9a, biological rep 1, technical rep 3 |
| GSM644215 |
Cybrid from haplogroup N9a, biological rep 1, technical rep 4 |
| GSM644216 |
Cybrid from haplogroup N9a, biological rep 2, technical rep 1 |
| GSM644217 |
Cybrid from haplogroup N9a, biological rep 2, technical rep 2 |
| GSM644218 |
Cybrid from haplogroup N9a, biological rep 2, technical rep 3 |
| GSM644219 |
Cybrid from haplogroup N9a, biological rep 2, technical rep 4 |
| GSM644220 |
Cybrid from haplogroup N9a, biological rep 3, technical rep 1 |
| GSM644221 |
Cybrid from haplogroup N9a, biological rep 3, technical rep 2 |
| GSM644222 |
Cybrid from haplogroup N9a, biological rep 3, technical rep 3 |
| GSM644223 |
Cybrid from haplogroup N9a, biological rep 3, technical rep 4 |
| GSM644224 |
Cybrid from haplogroup N9a, biological rep 4, technical rep 1 |
| GSM644225 |
Cybrid from haplogroup N9a, biological rep 4, technical rep 2 |
| GSM644226 |
Cybrid from haplogroup N9a, biological rep 4, technical rep 3 |
| GSM644227 |
Cybrid from haplogroup N9a, biological rep 4, technical rep 4 |
| GSM644228 |
rho0 cell, technical rep 1 |
| GSM644229 |
rho0 cell, technical rep 2 |
| GSM644230 |
rho0 cell, technical rep 3 |
| GSM644231 |
rho0 cell, technical rep 4 |
| GSM644232 |
rho0 cell, technical rep 5 |
| GSM644233 |
rho0 cell, technical rep 6 |
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| Relations |
| BioProject |
PRJNA135073 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26244_RAW.tar |
224.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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