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Series GSE26309 Query DataSets for GSE26309
Status Public on Jan 02, 2011
Title A Transcriptomic Analysis of NET1 (a RhoA GEF Exchange Factor) in AGS Gastric Cancer Cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Stable knockdown of NET1, a RhoGEF, was achieved in AGS Gastric Cancer cells. This gene is known to be overexpressed in the disease.
Knockdown was achieved using lentiviral shRNA particles. Gene expression was compared between knockdown and scrambled shRNA treated control cells.
Cells were treated with and without LPA, a known activator of RhoA.
 
Overall design Three distinct cell lines were used in this study (all AGS cells); (i) Non Target cell (NT) stably expressing non targetting shRNA (ii) 63 and (iii) 65; the latter two are stable NET1 knockdown cells and are seperatly transduced with separate NET1 targetting shRNA particles. Cells were treated with and without 10microM LPA for 4 hr. Experimental replicates were performed for each treatment (A & B), RNA was prepared from each and seperatly hybridised to U133A arrays.
 
Contributor(s) Murray D
Citation(s) 21284875
Submission date Dec 27, 2010
Last update date Dec 06, 2018
Contact name David MURRAY
Organization name RCSI
Street address 123 St Stephens Green
City Dublin
ZIP/Postal code D2
Country Ireland
 
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (12)
GSM646065 GB 1 63A +LPA
GSM646066 GB 2 63B +LPA
GSM646067 GB 3 65A +LPA
Relations
BioProject PRJNA136751

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26309_RAW.tar 23.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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